Rapid Detection of Methicillin-resistant Staphylococcus Aureus (MRSA) in Diverse Clinical Specimens by the BD GeneOhm MRSA Assay and Comparison with Culture
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The efficacy of the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) assay was assessed by analyzing nasal swabs and swabs from other body sites for the presence of MRSA in a low-prevalence area. From 681 patients with a high risk for MRSA carriage, 1,601 specimens were collected and transported in Amies agar. After discordant analysis, the sensitivity, specificity, positive predictive value, and negative predictive value of the BD GeneOhm MRSA assay were 84.3%, 99.2%, 88.4%, and 98.9%, respectively, compared to culture.
PCR-based Approaches for the Detection of Clinical Methicillin-resistant Staphylococcus aureus.
Liu Y, Zhang J, Ji Y Open Microbiol J. 2016; 10:45-56.
PMID: 27335617 PMC: 4899539. DOI: 10.2174/1874285801610010045.
Schinasi L, Wing S, Augustino K, Ramsey K, Nobles D, Richardson D Environ Health. 2014; 13(1):54.
PMID: 24958086 PMC: 4083368. DOI: 10.1186/1476-069X-13-54.
Schinasi L, Wing S, MacDonald P, Richardson D, Stewart J, Augustino K PLoS One. 2013; 8(8):e73595.
PMID: 23991200 PMC: 3753306. DOI: 10.1371/journal.pone.0073595.
Analysis of Staphylococcal cassette chromosome mec in BD GeneOhm MRSA assay-negative strains.
Zhang M, Ito T, Li S, Misawa S, Kondo S, Miida T Antimicrob Agents Chemother. 2013; 57(6):2890-1.
PMID: 23571551 PMC: 3716159. DOI: 10.1128/AAC.00174-13.
Tubbicke A, Hubner C, Kramer A, Hubner N, Flessa S Eur J Clin Microbiol Infect Dis. 2012; 31(10):2497-511.
PMID: 22573360 DOI: 10.1007/s10096-012-1632-8.