Nucleosome Remodeling by HMSH2-hMSH6

Overview

Journal Mol Cell

Publisher Cell Press

Specialty Cell Biology

Date 2010 Jan 13

PMID 20064472

Citations 52

Authors

Sarah Javaid

Mridula Manohar

Nidhi Punja

Alex Mooney

Jennifer J Ottesen

Michael G Poirier

Richard Fishel

Affiliations

Soon will be listed here.

Abstract

DNA nucleotide mismatches and lesions arise on chromosomes that are a complex assortment of protein and DNA (chromatin). The fundamental unit of chromatin is a nucleosome that contains approximately 146 bp DNA wrapped around an H2A, H2B, H3, and H4 histone octamer. We demonstrate that the mismatch recognition heterodimer hMSH2-hMSH6 disassembles a nucleosome. Disassembly requires a mismatch that provokes the formation of hMSH2-hMSH6 hydrolysis-independent sliding clamps, which translocate along the DNA to the nucleosome. The rate of disassembly is enhanced by actual or mimicked acetylation of histone H3 within the nucleosome entry-exit and dyad axis that occurs during replication and repair in vivo and reduces DNA-octamer affinity in vitro. Our results support a passive mechanism for chromatin remodeling whereby hMSH2-hMSH6 sliding clamps trap localized fluctuations in nucleosome positioning and/or wrapping that ultimately leads to disassembly, and highlight unanticipated strengths of the Molecular Switch Model for mismatch repair (MMR).

Citing Articles

MutS functions as a clamp loader by positioning MutL on the DNA during mismatch repair.

Yang X, Han X, Han C, London J, Fishel R, Liu J Nat Commun. 2022; 13(1):5808.

PMID: 36192430 PMC: 9530208. DOI: 10.1038/s41467-022-33479-3.

Retroviral prototype foamy virus intasome binding to a nucleosome target does not determine integration efficiency.

Kotlar R, Jones N, Senavirathne G, Gardner A, Messer R, Tan Y J Biol Chem. 2021; 296:100550.

PMID: 33744295 PMC: 8050864. DOI: 10.1016/j.jbc.2021.100550.

Chromatin remodeling and mismatch repair: Access and excision.

Goellner E DNA Repair (Amst). 2019; 85:102733.

PMID: 31698199 PMC: 7367450. DOI: 10.1016/j.dnarep.2019.102733.

Nucleosome DNA unwrapping does not affect prototype foamy virus integration efficiency or site selection.

Mackler R, Jones N, Gardner A, Lopez Jr M, Howard C, Fishel R PLoS One. 2019; 14(3):e0212764.

PMID: 30865665 PMC: 6415784. DOI: 10.1371/journal.pone.0212764.

The properties of Msh2-Msh6 ATP binding mutants suggest a signal amplification mechanism in DNA mismatch repair.

Graham 5th W, Putnam C, Kolodner R J Biol Chem. 2018; 293(47):18055-18070.

PMID: 30237169 PMC: 6254361. DOI: 10.1074/jbc.RA118.005439.

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