The DEAD-box RNA Helicase Ded1p Affects and Accumulates in Saccharomyces Cerevisiae P-bodies

Overview

Journal Mol Biol Cell

Specialties Cell Biology
Molecular Biology

Date 2007 Dec 29

PMID 18162578

Citations 63

Authors

Carla Beckham

Angela Hilliker

Anne-Marie Cziko

Amine Noueiry

Mani Ramaswami

Roy Parker

Affiliations

Soon will be listed here.

Abstract

Recent results suggest that cytoplasmic mRNAs can form translationally repressed messenger ribonucleoprotein particles (mRNPs) capable of decapping and degradation, or accumulation into cytoplasmic processing bodies (P-bodies), which can function as sites of mRNA storage. The proteins that function in transitions between the translationally repressed mRNPs that accumulate in P-bodies and mRNPs engaged in translation are largely unknown. Herein, we demonstrate that the yeast translation initiation factor Ded1p can localize to P-bodies. Moreover, depletion of Ded1p leads to defects in P-body formation. Overexpression of Ded1p results in increased size and number of P-bodies and inhibition of growth in a manner partially suppressed by loss of Pat1p, Dhh1p, or Lsm1p. Mutations that inactivate the ATPase activity of Ded1p increase the overexpression growth inhibition of Ded1p and prevent Ded1p from localizing in P-bodies. Combined with earlier work showing Ded1p can have a positive effect on translation, these results suggest that Ded1p is a bifunctional protein that can affect both translation initiation and P-body formation.

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