Dissection of the Bifunctional ARGRII Protein Involved in the Regulation of Arginine Anabolic and Catabolic Pathways

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1991 Apr 1

PMID 2005903

Citations 27

Authors

H F Qui

E Dubois

F Messenguy

Affiliations

Soon will be listed here.

Abstract

ARGRII is a regulatory protein which regulates the arginine anabolic and catabolic pathways in combination with ARGRI and ARGRIII. We have investigated, by deletion analysis and fusion to LexA protein, the different domains of ARGRII protein. In contrast to other yeast regulatory proteins, 92% of ARGRII is necessary for its anabolic repression function and 80% is necessary for its catabolic activator function. We can define three domains in this protein: a putative DNA-binding domain containing a zinc finger motif, a region more involved in the repression activity located around the RNase-like sequence, and a large activation domain.

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