In Vitro Studies of the Binding of the ARGR Proteins to the ARG5,6 Promoter

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1991 Apr 1

PMID 2005902

Citations 28

Authors

E Dubois

F Messenguy

Affiliations

Soon will be listed here.

Abstract

ARGRI, ARGRII, and ARGRIII regulatory proteins control the expression of arginine anabolic and catabolic genes in Saccharomyces cerevisiae. We show here that they are also required in vitro to observe a protein-DNA complex with the promoter of the ARG5,6 gene. The specific binding of ARGR proteins in vitro is stimulated by arginine. Antibodies raised against a synthetic MCM1 polypeptide retard the migration of ARGR-DNA complex on gel mobility shift assays. This result suggests that MCM1 could be an additional regulatory element of arginine metabolism.

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