High-throughput Caveolar Proteomic Signature Profile for Maternal Binge Alcohol Consumption

Overview

Journal Alcohol

Specialty Psychiatry

Date 2010 Jan 8

PMID 20053519

Citations 15

Authors

Jayanth Ramadoss

Wu-Xiang Liao

Dong-Bao Chen

Ronald R Magness

Affiliations

Soon will be listed here.

Abstract

Currently, no single marker is sensitive and specific enough to be considered a reliable biomarker for prenatal alcohol exposure. To identify a proteomic signature profile for maternal alcohol consumption, we carried out high-throughput proteomics on maternal endothelial caveolae exposed to moderate binge-like alcohol conditions. In these specialized lipid-ordered microdomains that contain a rich assembly of proteins, we demonstrate that moderate binge-like alcohol resulted in a distinctive maternal caveolar proteomic signature with important proteins being dramatically decreased/knocked out in the alcoholic profile. These proteins span from histones and basic structural proteins like α tubulin to proteins involved in trafficking, deubiquitination, cell signaling, and cell-cell adhesion. The profile also suggests an important role for the mother and the uteroplacental compartment in the pathogenesis of fetal alcohol spectrum disorders (FASD). These data demonstrate that the caveolar proteomic signature created by alcohol shows a promising direction for early detection of FASD.

Citing Articles

Fetal regional brain protein signature in FASD rat model.

Davis-Anderson K, Wesseling H, Siebert L, Lunde-Young E, Naik V, Steen H Reprod Toxicol. 2018; 76:84-92.

PMID: 29408587 PMC: 5834402. DOI: 10.1016/j.reprotox.2018.01.004.

Epigenetics studies of fetal alcohol spectrum disorder: where are we now?.

Lussier A, Weinberg J, Kobor M Epigenomics. 2017; 9(3):291-311.

PMID: 28234026 PMC: 5549650. DOI: 10.2217/epi-2016-0163.

Domain-Specific Partitioning of Uterine Artery Endothelial Connexin43 and Caveolin-1.

Ampey B, Morschauser T, Ramadoss J, Magness R Hypertension. 2016; 68(4):982-8.

PMID: 27572151 PMC: 5016248. DOI: 10.1161/HYPERTENSIONAHA.116.08000.

Epigenetic Mechanisms in Developmental Alcohol-Induced Neurobehavioral Deficits.

Basavarajappa B, Subbanna S Brain Sci. 2016; 6(2).

PMID: 27070644 PMC: 4931489. DOI: 10.3390/brainsci6020012.

A comparison of the different animal models of fetal alcohol spectrum disorders and their use in studying complex behaviors.

Patten A, Fontaine C, Christie B Front Pediatr. 2014; 2:93.

PMID: 25232537 PMC: 4153370. DOI: 10.3389/fped.2014.00093.

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