Alcohol-induced Alterations in Maternal Uterine Endothelial Proteome: a Quantitative ITRAQ Mass Spectrometric Approach

Overview

Journal Reprod Toxicol

Specialties Reproductive Medicine
Toxicology

Date 2012 Sep 11

PMID 22960358

Citations 16

Authors

Jayanth Ramadoss

Ronald R Magness

Affiliations

Soon will be listed here.

Abstract

Objective: To quantitate alcohol-induced alterations in the maternal uterine endothelial proteome utilizing iTRAQ-based mass spectrometry.

Study Design: Uterine artery endothelial cells from third trimester pregnant ewes were FAC sorted, validated and treated without or with binge-like alcohol. Lysates were trypsin digested, iTRAQ-labeled, and analyzed using nano LC MS/MS.

Results: Alcohol significantly upregulated 14 and downregulated 17 proteins (P<0.05) including those related to cell structure, transcription/translation regulation, histones, Ca(2+)/NO, and redox balance. Gene Ontology and ArrayTrack analyses revealed alterations to protein processing, binding, and nutrient metabolism pathways. Further, alcohol altered proteins previously correlated with fetal alcohol spectrum disorders (FASD) and those that regulate epigenetic, transcriptional, and translational processes.

Conclusions: Alcohol differentially alters the proteome in the maternal uterine compartment at the level of the endothelium. iTRAQ mass spectrometry provides a robust high throughput platform to comprehend the multi-mechanistic actions of alcohol and develop appropriate biomarkers and ameliorative measures for FASD.

Citing Articles

In Vivo Administration of Phosphatidic Acid, a Direct Alcohol Target Rescues Fetal Growth Restriction and Maternal Uterine Artery Dysfunction in Rat FASD Model.

Janeski J, Naik V, Carabulea A, Jiang H, Ramadoss J Nutrients. 2024; 16(10).

PMID: 38794647 PMC: 11123873. DOI: 10.3390/nu16101409.

Involvement of metalloproteinase and nitric oxide synthase/nitric oxide mechanisms in early decidual angiogenesis-vascularization of normal and experimental pathological mouse placenta related to maternal alcohol exposure.

Gualdoni G, Barril C, Jacobo P, Pacheco Rodriguez L, Cebral E Front Cell Dev Biol. 2023; 11:1207671.

PMID: 37670932 PMC: 10476144. DOI: 10.3389/fcell.2023.1207671.

Early Abnormal Placentation and Evidence of Vascular Endothelial Growth Factor System Dysregulation at the Feto-Maternal Interface After Periconceptional Alcohol Consumption.

Gualdoni G, Jacobo P, Barril C, Ventureira M, Cebral E Front Physiol. 2022; 12:815760.

PMID: 35185604 PMC: 8847216. DOI: 10.3389/fphys.2021.815760.

Ethanol-Induced Alterations in Placental and Fetal Cerebrocortical Annexin-A4 and Cerebral Cavernous Malformation Protein 3 Are Associated With Reductions in Fetal Cortical VEGF Receptor Binding and Microvascular Density.

Savage D, Rosenberg M, Coquet L, Porch M, Allen N, Roux C Front Neurosci. 2020; 14:519.

PMID: 32655346 PMC: 7325964. DOI: 10.3389/fnins.2020.00519.

Gestational binge alcohol-induced alterations in maternal uterine artery transcriptome.

Orzabal M, Lunde-Young E, Ramirez J, Naik V, Hillhouse A, Konganti K Reprod Toxicol. 2019; 87:42-49.

PMID: 31078653 PMC: 6628922. DOI: 10.1016/j.reprotox.2019.05.053.

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