» Articles » PMID: 20039198

Cumulus Cell Contact During Oocyte Maturation in Mice Regulates Meiotic Spindle Positioning and Enhances Developmental Competence

Overview
Publisher Springer
Date 2009 Dec 30
PMID 20039198
Citations 34
Authors
Affiliations
Soon will be listed here.
Abstract

Purpose: To investigate the role of cumulus cell contact during oocyte maturation on meiotic spindle assembly and the acquisition of developmental competence.

Methods: Cumulus oocyte complexes isolated from mouse ovaries subjected to in vitro or in vivo maturation were analyzed by confocal microscopy with respect to oocyte somatic cell contacts and for their ability to develop after parthenogenic activation during embryo culture.

Results: Cell contact is maintained during maturation in vivo, predisposing oocytes to cortical meiotic spindle assembly and developmental competence acquisition. In contrast, oocytes matured in vitro lose cell contact coincident with central meiotic spindle assembly that results in cleavage delays upon egg activation and failure to form blastocysts. Experimental disruption of cell contact by the actin-depolymerizing agent latrunculin B results in the formation of enlarged meiotic spindles with dispersed chromosomes unlike the compact ordering of chromosomes observed on spindles formed after in vivo maturation, suggesting a link between cell contact and the acquisition of developmental competence.

Conclusions: Somatic cell contact optimizes oocyte quality during meiotic maturation by regulating the spatial organization and function of the meiotic spindle through actin-dependent mechanisms that enhance development.

Citing Articles

Luteinizing Hormone Receptor Mutation (LHR) Causes Abnormal Follicular Development Revealed by Follicle Single-Cell Analysis and CRISPR/Cas9.

Zhang C, Nie Y, Xu B, Mu C, Tian G, Li X Interdiscip Sci. 2024; 16(4):976-989.

PMID: 39150470 PMC: 11512921. DOI: 10.1007/s12539-024-00646-7.


Developmental perturbation in human embryos: Clinical and biological significance learned from time-lapse images.

Ezoe K, Takahashi T, Miki T, Kato K Reprod Med Biol. 2024; 23(1):e12593.

PMID: 38983691 PMC: 11232294. DOI: 10.1002/rmb2.12593.


Reproductive Ageing: Metabolic contribution to age-related chromosome missegregation in mammalian oocytes.

Mihalas B, Marston A, Wu L, Gilchrist R Reproduction. 2024; 168(2).

PMID: 38718822 PMC: 11301428. DOI: 10.1530/REP-23-0510.


Making a good egg: human oocyte health, aging, and in vitro development.

Telfer E, Grosbois J, Odey Y, Rosario R, Anderson R Physiol Rev. 2023; 103(4):2623-2677.

PMID: 37171807 PMC: 10625843. DOI: 10.1152/physrev.00032.2022.


Morphokinetic parameters of mouse oocyte meiotic maturation and cumulus expansion are not affected by reproductive age or ploidy status.

Suebthawinkul C, Babayev E, Lee H, Duncan F J Assist Reprod Genet. 2023; 40(5):1197-1213.

PMID: 37012451 PMC: 10239409. DOI: 10.1007/s10815-023-02779-y.


References
1.
Verlhac M, Lefebvre C, Guillaud P, Rassinier P, Maro B . Asymmetric division in mouse oocytes: with or without Mos. Curr Biol. 2000; 10(20):1303-6. DOI: 10.1016/s0960-9822(00)00753-3. View

2.
Plancha C, Albertini D . Hormonal regulation of meiotic maturation in the hamster oocyte involves a cytoskeleton-mediated process. Biol Reprod. 1994; 51(5):852-64. DOI: 10.1095/biolreprod51.5.852. View

3.
Elvin J, Clark A, Wang P, Wolfman N, Matzuk M . Paracrine actions of growth differentiation factor-9 in the mammalian ovary. Mol Endocrinol. 1999; 13(6):1035-48. DOI: 10.1210/mend.13.6.0310. View

4.
Azoury J, Lee K, Georget V, Rassinier P, Leader B, Verlhac M . Spindle positioning in mouse oocytes relies on a dynamic meshwork of actin filaments. Curr Biol. 2008; 18(19):1514-9. DOI: 10.1016/j.cub.2008.08.044. View

5.
Gilchrist R, Lane M, Thompson J . Oocyte-secreted factors: regulators of cumulus cell function and oocyte quality. Hum Reprod Update. 2008; 14(2):159-77. DOI: 10.1093/humupd/dmm040. View