Genetic and Genomic Dissection of Cardiogenesis in the Drosophila Model

Overview

Journal Pediatr Cardiol

Specialties Cardiology & Vascular Diseases
Pediatrics

Date 2009 Dec 25

PMID 20033682

Citations 18

Authors

Ingolf Reim

Manfred Frasch

Affiliations

Soon will be listed here.

Abstract

The linear heart tube of the fruit fly Drosophila has served as a very valuable model for studying the regulation of early heart development. In the past, regulatory genes of Drosophila cardiogenesis have been identified largely through candidate approaches. The vast genetic toolkit available in this organism has made it possible to determine their functions and build regulatory networks of transcription factors and signaling inputs that control heart development. In this review, we summarize the major findings from this study and present current approaches aiming to identify additional players in the specification, morphogenesis, and differentiation of the heart by forward genetic screens. We also discuss various genomic and bioinformatic approaches that are currently being developed to extend the known transcriptional networks more globally which, in combination with the genetic approaches, will provide a comprehensive picture of the regulatory circuits during cardiogenesis.

Citing Articles

From promoter motif to cardiac function: a single DPE motif affects transcription regulation and organ function in vivo.

Sloutskin A, Itzhak D, Vogler G, Pozeilov H, Ideses D, Alter H Development. 2024; 151(14).

PMID: 38958007 PMC: 11317100. DOI: 10.1242/dev.202355.

Technologies to Study Genetics and Molecular Pathways.

Grunert M, Dorn C, Dopazo A, Sanchez-Cabo F, Vazquez J, Rickert-Sperling S Adv Exp Med Biol. 2024; 1441:435-458.

PMID: 38884724 DOI: 10.1007/978-3-031-44087-8_22.

Single-cell profiling of the developing embryonic heart in Drosophila.

Huang X, Fu Y, Lee H, Zhao Y, Yang W, van de Leemput J Development. 2023; 150(16).

PMID: 37526610 PMC: 10482008. DOI: 10.1242/dev.201936.

Conserved chamber-specific polyploidy maintains heart function in Drosophila.

Chakraborty A, Peterson N, King J, Gross R, Mendiola Pla M, Thennavan A Development. 2023; 150(16).

PMID: 37526609 PMC: 10482010. DOI: 10.1242/dev.201896.

Simultaneous cellular and molecular phenotyping of embryonic mutants using single-cell regulatory trajectories.

Secchia S, Forneris M, Heinen T, Stegle O, Furlong E Dev Cell. 2022; 57(4):496-511.e8.

PMID: 35176234 PMC: 8893321. DOI: 10.1016/j.devcel.2022.01.016.

References

Gajewski K, Zhang Q, Choi C, Fossett N, Dang A, Kim Y . Pannier is a transcriptional target and partner of Tinman during Drosophila cardiogenesis. Dev Biol. 2001; 233(2):425-36. DOI: 10.1006/dbio.2001.0220. View

Philippakis A, Busser B, Gisselbrecht S, He F, Estrada B, Michelson A . Expression-guided in silico evaluation of candidate cis regulatory codes for Drosophila muscle founder cells. PLoS Comput Biol. 2006; 2(5):e53. PMC: 1464814. DOI: 10.1371/journal.pcbi.0020053. View

Gajewski K, Choi C, Kim Y, Schulz R . Genetically distinct cardial cells within the Drosophila heart. Genesis. 2000; 28(1):36-43. DOI: 10.1002/1526-968x(200009)28:1<36::aid-gene50>3.0.co;2-4. View

Bozkulak E, Weinmaster G . Selective use of ADAM10 and ADAM17 in activation of Notch1 signaling. Mol Cell Biol. 2009; 29(21):5679-95. PMC: 2772745. DOI: 10.1128/MCB.00406-09. View

Monier B, Astier M, Semeriva M, Perrin L . Steroid-dependent modification of Hox function drives myocyte reprogramming in the Drosophila heart. Development. 2005; 132(23):5283-93. DOI: 10.1242/dev.02091. View

Segal E, Raveh-Sadka T, Schroeder M, Unnerstall U, Gaul U . Predicting expression patterns from regulatory sequence in Drosophila segmentation. Nature. 2008; 451(7178):535-40. DOI: 10.1038/nature06496. View

Lours C, Bardot O, Godt D, Laski F, Couderc J . The Drosophila melanogaster BTB proteins bric à brac bind DNA through a composite DNA binding domain containing a pipsqueak and an AT-Hook motif. Nucleic Acids Res. 2003; 31(18):5389-98. PMC: 203310. DOI: 10.1093/nar/gkg724. View

Sellin J, Albrecht S, Kolsch V, Paululat A . Dynamics of heart differentiation, visualized utilizing heart enhancer elements of the Drosophila melanogaster bHLH transcription factor Hand. Gene Expr Patterns. 2006; 6(4):360-75. DOI: 10.1016/j.modgep.2005.09.012. View

Yin Z, Frasch M . Regulation and function of tinman during dorsal mesoderm induction and heart specification in Drosophila. Dev Genet. 1998; 22(3):187-200. DOI: 10.1002/(SICI)1520-6408(1998)22:3<187::AID-DVG2>3.0.CO;2-2. View

10.

Han Z, Bodmer R . Myogenic cells fates are antagonized by Notch only in asymmetric lineages of the Drosophila heart, with or without cell division. Development. 2003; 130(13):3039-51. DOI: 10.1242/dev.00484. View

11.

Kolsch V, Paululat A . The highly conserved cardiogenic bHLH factor Hand is specifically expressed in circular visceral muscle progenitor cells and in all cell types of the dorsal vessel during Drosophila embryogenesis. Dev Genes Evol. 2002; 212(10):473-85. DOI: 10.1007/s00427-002-0268-6. View

12.

Zeitouni B, Senatore S, Severac D, Aknin C, Semeriva M, Perrin L . Signalling pathways involved in adult heart formation revealed by gene expression profiling in Drosophila. PLoS Genet. 2007; 3(10):1907-21. PMC: 2014791. DOI: 10.1371/journal.pgen.0030174. View

13.

Reim I, Lee H, Frasch M . The T-box-encoding Dorsocross genes function in amnioserosa development and the patterning of the dorsolateral germ band downstream of Dpp. Development. 2003; 130(14):3187-204. DOI: 10.1242/dev.00548. View

14.

Akazawa H, Komuro I . Cardiac transcription factor Csx/Nkx2-5: Its role in cardiac development and diseases. Pharmacol Ther. 2005; 107(2):252-68. DOI: 10.1016/j.pharmthera.2005.03.005. View

15.

Halfon M, Grad Y, Church G, Michelson A . Computation-based discovery of related transcriptional regulatory modules and motifs using an experimentally validated combinatorial model. Genome Res. 2002; 12(7):1019-28. PMC: 186630. DOI: 10.1101/gr.228902. View

16.

Mann T, Bodmer R, Pandur P . The Drosophila homolog of vertebrate Islet1 is a key component in early cardiogenesis. Development. 2008; 136(2):317-26. PMC: 2685972. DOI: 10.1242/dev.022533. View

17.

Tomancak P, Beaton A, Weiszmann R, Kwan E, Shu S, Lewis S . Systematic determination of patterns of gene expression during Drosophila embryogenesis. Genome Biol. 2003; 3(12):RESEARCH0088. PMC: 151190. DOI: 10.1186/gb-2002-3-12-research0088. View

18.

Lo P, Frasch M . Establishing A-P polarity in the embryonic heart tube: a conserved function of Hox genes in Drosophila and vertebrates?. Trends Cardiovasc Med. 2003; 13(5):182-7. DOI: 10.1016/s1050-1738(03)00074-4. View

19.

Monier B, Tevy M, Perrin L, Capovilla M, Semeriva M . Downstream of homeotic genes: in the heart of Hox function. Fly (Austin). 2008; 1(2):59-67. DOI: 10.4161/fly.3993. View

20.

Reim I, Mohler J, Frasch M . Tbx20-related genes, mid and H15, are required for tinman expression, proper patterning, and normal differentiation of cardioblasts in Drosophila. Mech Dev. 2005; 122(9):1056-69. DOI: 10.1016/j.mod.2005.04.006. View