Chronic Exposure to Zinc Chromate Induces Centrosome Amplification and Spindle Assembly Checkpoint Bypass in Human Lung Fibroblasts

Overview

Journal Chem Res Toxicol

Publisher American Chemical Society

Specialty Toxicology

Date 2009 Dec 25

PMID 20030412

Citations 27

Authors

Amie L Holmes

Sandra S Wise

Stephen C Pelsue

AbouEl-Makarim Aboueissa

Wilma Lingle

Jeffery Salisbury

Jamie Gallagher

John Pierce Wise Sr

Affiliations

Soon will be listed here.

Abstract

Hexavalent chromium (Cr(VI)) compounds are known human lung carcinogens. Solubility plays an important role in its carcinogenicity with the particulate or insoluble form being the most potent. Of the particulate Cr(VI) compounds, zinc chromate appears to be the most potent carcinogen; however, very few studies have investigated its carcinogenic mechanism. In this study, we investigated the ability of chronic exposure to zinc chromate to induce numerical chromosome instability. We found no increase in aneuploidy after a 24 h exposure to zinc chromate, but with more chronic exposures, zinc chromate induced concentration- and time-dependent increases in aneuploidy in the form of hypodiploidy, hyperdiploidy, and tetraploidy. Zinc chromate also induced centrosome amplification in a concentration- and time-dependent manner in both interphase and mitotic cells after chronic exposure, producing cells with centriolar defects. Furthermore, chronic exposure to zinc chromate induced concentration- and time-dependent increases in spindle assembly checkpoint bypass with increases in centromere spreading, premature centromere division, and premature anaphase. Last, we found that chronic exposure to zinc chromate induced a G2 arrest. All together, these data indicate that zinc chromate can induce chromosome instability after prolonged exposures.

Citing Articles

Carcinogenic Mechanisms of Hexavalent Chromium: From DNA Breaks to Chromosome Instability and Neoplastic Transformation.

Meaza I, Williams A, Wise S, Lu H, Pierce Sr J, Wise Sr J Curr Environ Health Rep. 2024; 11(4):484-546.

PMID: 39466546 PMC: 11872169. DOI: 10.1007/s40572-024-00460-9.

Chromate Affects Gene Expression and DNA Methylation in Long-Term In Vitro Experiments in A549 Cells.

Fischer F, Stosser S, Wegmann L, Veh E, Lumpp T, Parsdorfer M Int J Mol Sci. 2024; 25(18).

PMID: 39337613 PMC: 11431867. DOI: 10.3390/ijms251810129.

A whale of a tale: whale cells evade the driving mechanism for hexavalent chromium-induced chromosome instability.

Lu H, Toyoda J, Wise S, Browning C, Speer R, Croom-Perez T Toxicol Sci. 2024; 199(1):49-62.

PMID: 38539048 PMC: 11057468. DOI: 10.1093/toxsci/kfae030.

Hexavalent Chromium Targets Securin to Drive Numerical Chromosome Instability in Human Lung Cells.

Toyoda J, Martino J, Speer R, Meaza I, Lu H, Williams A Int J Mol Sci. 2024; 25(1).

PMID: 38203427 PMC: 10778806. DOI: 10.3390/ijms25010256.

Inflammatory effects of hexavalent chromium in the lung: A comprehensive review.

Kouokam J, Meaza I, Wise Sr J Toxicol Appl Pharmacol. 2022; 455:116265.

PMID: 36208701 PMC: 10024459. DOI: 10.1016/j.taap.2022.116265.

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