» Articles » PMID: 2001788

Cell Culture Models of Differentiation

Overview
Journal FASEB J
Specialties Biology
Physiology
Date 1991 Mar 1
PMID 2001788
Citations 10
Authors
Affiliations
Soon will be listed here.
Abstract

It is now possible to culture cells from most organs of the body under conditions in which they continue to express at least some of their differentiated traits, and to model some of the differentiation processes that occur during embryonic and adult life. How much can these cultures tell us about the acquisition and maintenance of the differentiated state? To answer this question I shall outline the features of several cell culture models, dividing them into categories according to whether they mimic differentiation during development, differentiation of adult stem cell progeny, or the transition from one differentiated phenotype to another. In spite of the diversity of cell types under consideration, it is possible to detect some common themes: the stability of the differentiated state; the relationship between proliferation and differentiation; the relative importance of intrinsic cellular programming and environmental regulation; and possible mechanisms for transcriptional control of the genes that are activated during differentiation. In recent years cell culture models have yielded a great deal of information about differentiation and the way is now clear for even more exciting discoveries.

Citing Articles

Maintenance of human hepatocyte function in vitro by liver-derived extracellular matrix gels.

Sellaro T, Ranade A, Faulk D, McCabe G, Dorko K, Badylak S Tissue Eng Part A. 2009; 16(3):1075-82.

PMID: 19845461 PMC: 2863084. DOI: 10.1089/ten.TEA.2008.0587.


Human umbilical cord blood-derived mast cells: a unique model for the study of neuro-immuno-endocrine interactions.

Theoharides T, Kempuraj D, Tagen M, Vasiadi M, Cetrulo C Stem Cell Rev. 2007; 2(2):143-54.

PMID: 17237553 DOI: 10.1007/s12015-006-0021-z.


Whole-body protochordate regeneration from totipotent blood cells.

Rinkevich B, Shlemberg Z, Fishelson L Proc Natl Acad Sci U S A. 1995; 92(17):7695-9.

PMID: 11607571 PMC: 41212. DOI: 10.1073/pnas.92.17.7695.


Oncogenic transformation and inhibition of adipocytic conversion of preadipocytes by TLS/FUS-CHOP type II chimeric protein.

Kuroda M, Ishida T, Takanashi M, Satoh M, Machinami R, Watanabe T Am J Pathol. 1997; 151(3):735-44.

PMID: 9284822 PMC: 1857848.


Correct cell- and differentiation-specific expression of a murine alpha 1 (I) collagen minigene in vitro differentiating embryonal carcinoma cells.

Rhodes K, Hall K, Lee K, Razzaghi H, Breindl M Gene Expr. 1996; 6(1):35-44.

PMID: 8931990 PMC: 6148262.