T-lymphocyte Interleukin 2-dependent Tyrosine Protein Kinase Signal Transduction Involves the Activation of P56lck

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1991 Mar 1

PMID 2000405

Citations 59

Authors

I D Horak

R E Gress

P J Lucas

E M Horak

T A Waldmann

J B Bolen

Affiliations

Soon will be listed here.

Abstract

Addition of interleukin 2 (IL-2) to IL-2-dependent T cells results in tyrosine protein kinase signal transduction events even though the IL-2 receptor alpha and beta chains lack intrinsic enzymatic activity. Here we report that addition of IL-2 to IL-2-dependent human T cells transiently stimulates the specific activity of p56lck, a member of the src family of nonreceptor tyrosine protein kinases expressed at high levels in T lymphocytes. The ability of IL-2 to induce p56lck activation was found to be independent of the capacity of p56lck to associate with either CD4 or CD8. Following IL-2 treatment, p56lck was found to undergo serine/threonine phosphorylation modifications that resulted in altered mobility of the lck gene product on polyacrylamide gels. These observations raise the possibility that p56lck participates in IL-2-mediated signal transduction events in T cells.

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