Interstrand Cross-links Are Preferentially Formed at the D(GC) Sites in the Reaction Between Cis-diamminedichloroplatinum (II) and DNA

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1991 Mar 1

PMID 2000402

Citations 61

Authors

M A Lemaire

A Schwartz

A R Rahmouni

M Leng

Affiliations

Soon will be listed here.

Abstract

A DNA restriction fragment with convergent SP6 and T7 promoters has undergone reaction with cis-diamminedichloroplatinum(II) (cis-DDP) and was then used as a template for RNA synthesis in vitro. The T7 and SP6 RNA polymerases generate fragments of defined sizes. Analysis of the RNA fragments shows that the polymerases are mainly blocked at the level of the d(GG) and d(AG) sites and to a lesser extent at the level of the d(GC) sites. The adducts at the d(GC) sites are more resistant to cyanide ion attack than those at the major sites and are identified as interstrand cross-links. The formation of an interstrand cross-link between the N-7 atoms of two guanine residues at the d(GC) sites was further confirmed by chemical modifications.

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References

Htun H, Dahlberg J . Single strands, triple strands, and kinks in H-DNA. Science. 1988; 241(4874):1791-6. DOI: 10.1126/science.3175620. View

Sorenson C, Eastman A . Mechanism of cis-diamminedichloroplatinum(II)-induced cytotoxicity: role of G2 arrest and DNA double-strand breaks. Cancer Res. 1988; 48(16):4484-8. View

Hemminki K, Thilly W . Binding of cisplatin to specific sequences of human DNA in vitro. Mutat Res. 1988; 202(1):133-8. DOI: 10.1016/0027-5107(88)90174-1. View

Marrot L, Leng M . Chemical probes of the conformation of DNA modified by cis-diamminedichloroplatinum(II). Biochemistry. 1989; 28(4):1454-61. DOI: 10.1021/bi00430a005. View

Corda Y, Job C, Anin M, Leng M, Job D . Transcription by eucaryotic and procaryotic RNA polymerases of DNA modified at a d(GG) or a d(AG) site by the antitumor drug cis-diamminedichloroplatinum(II). Biochemistry. 1991; 30(1):222-30. DOI: 10.1021/bi00215a032. View

Maxam A, Gilbert W . A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977; 74(2):560-4. PMC: 392330. DOI: 10.1073/pnas.74.2.560. View

Lippard S, Hoeschele J . Binding of cis- and trans-dichlorodiammineplatinum(II) to the nucleosome core. Proc Natl Acad Sci U S A. 1979; 76(12):6091-5. PMC: 411808. DOI: 10.1073/pnas.76.12.6091. View

CASTLEMAN H, Hanau L, Erlanger B . Stabilization of (dG-dC)n.(dG-dC)n in the Z conformation by a crosslinking reaction. Nucleic Acids Res. 1983; 11(23):8421-9. PMC: 326592. DOI: 10.1093/nar/11.23.8421. View

Pinto A, Lippard S . Sequence-dependent termination of in vitro DNA synthesis by cis- and trans-diamminedichloroplatinum (II). Proc Natl Acad Sci U S A. 1985; 82(14):4616-9. PMC: 390436. DOI: 10.1073/pnas.82.14.4616. View

10.

Eastman A . Interstrand cross-links and sequence specificity in the reaction of cis-dichloro(ethylenediamine)platinum(II) with DNA. Biochemistry. 1985; 24(19):5027-32. DOI: 10.1021/bi00340a011. View

11.

Axelrod V, Kramer F . Transcription from bacteriophage T7 and SP6 RNA polymerase promoters in the presence of 3'-deoxyribonucleoside 5'-triphosphate chain terminators. Biochemistry. 1985; 24(21):5716-23. DOI: 10.1021/bi00342a005. View

12.

Malinge J, Schwartz A, Leng M . Characterization of the ternary complexes formed in the reaction of cis-diamminedichloroplatinum (II), ethidium bromide and nucleic acids. Nucleic Acids Res. 1987; 15(4):1779-97. PMC: 340581. DOI: 10.1093/nar/15.4.1779. View

13.

Gralla J, Poljak L . Formation of blocking lesions at identical DNA sequences by the nitrosourea and platinum classes of anticancer drugs. Cancer Res. 1987; 47(19):5092-6. View

14.

Eastman A . The formation, isolation and characterization of DNA adducts produced by anticancer platinum complexes. Pharmacol Ther. 1987; 34(2):155-66. DOI: 10.1016/0163-7258(87)90009-x. View

15.

Villani G, Hubscher U, Butour J . Sites of termination of in vitro DNA synthesis on cis-diamminedichloroplatinum(II) treated single-stranded DNA: a comparison between E. coli DNA polymerase I and eucaryotic DNA polymerases alpha. Nucleic Acids Res. 1988; 16(10):4407-18. PMC: 336638. DOI: 10.1093/nar/16.10.4407. View

16.

Sorenson C, Eastman A . Influence of cis-diamminedichloroplatinum(II) on DNA synthesis and cell cycle progression in excision repair proficient and deficient Chinese hamster ovary cells. Cancer Res. 1988; 48(23):6703-7. View