Computational Identification and Experimental Validation of PPRE Motifs in NHE1 and MnSOD Genes of Human

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2009 Dec 5

PMID 19958503

Citations 22

Authors

Gireedhar Venkatachalam

Alan Prem Kumar

Loo Ser Yue

Shazib Pervaiz

Marie Veronique Clement

Meena Kishore Sakharkar

Affiliations

Soon will be listed here.

Abstract

Background: Activation of PPARs has been reported to inhibit the proliferation of malignant cells from different lineages. They are involved in transcription regulation of genes upon activation by a ligand. The binding of PPARs to the promoter sequence either represses or activates the gene. Hence, PPARs represent promising targets for cancer treatment because of their anti-proliferative and pro-apoptotic activities. Here we computationally identified PPAR binding regions in NHE1 and MnSOD. We further validated the predictions in vitro.

Results: Our results computationally predicted the presence of 2 PPRE motifs in NHE1 and 3 PPRE motifs in MnSOD. We experimentally confirmed the true motifs and their regulation by PPAR.

Conclusion: Our results suggest that both NHE1 and MnSOD have PPRE binding motif in their upstream/promoter region and hence are regulated by PPAR upon ligand binding.

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