Cryptosporidium Parvum Induces B7-H1 Expression in Cholangiocytes by Down-regulating MicroRNA-513

Overview

Journal J Infect Dis

Publisher Oxford University Press

Specialty Infectious Diseases

Date 2009 Nov 18

PMID 19916867

Citations 44

Authors

Ai-Yu Gong

Rui Zhou

Guoku Hu

Jun Liu

Danuta Sosnowska

Kristen M Drescher

Haidong Dong

Xian-Ming Chen

Affiliations

Soon will be listed here.

Abstract

Expression of B7 costimulatory molecules represents an important compartment of immune response of epithelial cells after microbial infection. We report here that the protozoan parasite Cryptosporidium parvum induced B7-H1 expression in cultured human cholangiocytes. Induced expression of B7-H1 was identified in cells after exposure to infective C. parvum parasite or parasite lysate. Interestingly, the level of microRNA-513 (miR-513) was reduced in cells after exposure to C. parvum, which resulted in a relief of 3' untranslated region-mediated translational suppression of B7-H1. Overexpression of miR-513 through transfection of miR-513 precursor inhibited C. parvum-induced B7-H1 protein expression. Moreover, enhanced apoptotic cell death was identified in activated human T cells after coculture with C. parvum-infected cholangiocytes. The apoptosis of activated T cells was partially blocked by a neutralizing antibody to B7-H1 or transfection of cholangiocytes with miR-513 precursor. These data suggest a role of miR-513 in regulating B7-H1 expression by cholangiocytes in response to C. parvum infection.

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References

Chen X, Splinter P, OHara S, LaRusso N . A cellular micro-RNA, let-7i, regulates Toll-like receptor 4 expression and contributes to cholangiocyte immune responses against Cryptosporidium parvum infection. J Biol Chem. 2007; 282(39):28929-28938. PMC: 2194650. DOI: 10.1074/jbc.M702633200. View

Miller T, Ghoshal K, Ramaswamy B, Roy S, Datta J, Shapiro C . MicroRNA-221/222 confers tamoxifen resistance in breast cancer by targeting p27Kip1. J Biol Chem. 2008; 283(44):29897-903. PMC: 2573063. DOI: 10.1074/jbc.M804612200. View

Yamazaki T, Akiba H, Iwai H, Matsuda H, Aoki M, Tanno Y . Expression of programmed death 1 ligands by murine T cells and APC. J Immunol. 2002; 169(10):5538-45. DOI: 10.4049/jimmunol.169.10.5538. View

Rogers K, Rogers A, Leav B, Sanchez A, Vannier E, Uematsu S . MyD88-dependent pathways mediate resistance to Cryptosporidium parvum infection in mice. Infect Immun. 2005; 74(1):549-56. PMC: 1346622. DOI: 10.1128/IAI.74.1.549-556.2006. View

Ledee N, Lombroso R, Lombardelli L, Selva J, Dubanchet S, Chaouat G . Cytokines and chemokines in follicular fluids and potential of the corresponding embryo: the role of granulocyte colony-stimulating factor. Hum Reprod. 2008; 23(9):2001-9. DOI: 10.1093/humrep/den192. View

Kamihira T, Shimoda S, Nakamura M, Yokoyama T, Takii Y, Kawano A . Biliary epithelial cells regulate autoreactive T cells: implications for biliary-specific diseases. Hepatology. 2004; 41(1):151-9. DOI: 10.1002/hep.20494. View

Ding H, Wu X, Gao W . PD-L1 is expressed by human renal tubular epithelial cells and suppresses T cell cytokine synthesis. Clin Immunol. 2005; 115(2):184-91. DOI: 10.1016/j.clim.2005.01.005. View

Strazzabosco M, Fabris L, Spirli C . Pathophysiology of cholangiopathies. J Clin Gastroenterol. 2005; 39(4 Suppl 2):S90-S102. DOI: 10.1097/01.mcg.0000155549.29643.ad. View

Chen X, OHara S, Nelson J, Splinter P, Small A, Tietz P . Multiple TLRs are expressed in human cholangiocytes and mediate host epithelial defense responses to Cryptosporidium parvum via activation of NF-kappaB. J Immunol. 2005; 175(11):7447-56. DOI: 10.4049/jimmunol.175.11.7447. View

10.

Chen X, Gores G, Paya C, Larusso N . Cryptosporidium parvum induces apoptosis in biliary epithelia by a Fas/Fas ligand-dependent mechanism. Am J Physiol. 1999; 277(3):G599-608. DOI: 10.1152/ajpgi.1999.277.3.G599. View

11.

Dong H, Zhu G, Tamada K, Flies D, van Deursen J, Chen L . B7-H1 determines accumulation and deletion of intrahepatic CD8(+) T lymphocytes. Immunity. 2004; 20(3):327-36. DOI: 10.1016/s1074-7613(04)00050-0. View

12.

Han J, Ulevitch R . Limiting inflammatory responses during activation of innate immunity. Nat Immunol. 2005; 6(12):1198-205. DOI: 10.1038/ni1274. View

13.

Grubman S, Perrone R, Lee D, Murray S, Rogers L, Wolkoff L . Regulation of intracellular pH by immortalized human intrahepatic biliary epithelial cell lines. Am J Physiol. 1994; 266(6 Pt 1):G1060-70. DOI: 10.1152/ajpgi.1994.266.6.G1060. View

14.

Viswanathan V, Hecht G . Innate immunity and the gut. Curr Opin Gastroenterol. 2006; 16(6):546-51. DOI: 10.1097/00001574-200011000-00015. View

15.

Pedersen I, Cheng G, Wieland S, Volinia S, Croce C, Chisari F . Interferon modulation of cellular microRNAs as an antiviral mechanism. Nature. 2007; 449(7164):919-22. PMC: 2748825. DOI: 10.1038/nature06205. View

16.

Wu H, Neilson J, Kumar P, Manocha M, Shankar P, Sharp P . miRNA profiling of naïve, effector and memory CD8 T cells. PLoS One. 2007; 2(10):e1020. PMC: 2000354. DOI: 10.1371/journal.pone.0001020. View

17.

McDonald V . Host cell-mediated responses to infection with Cryptosporidium. Parasite Immunol. 2000; 22(12):597-604. DOI: 10.1046/j.1365-3024.2000.00343.x. View

18.

Alpini G, McGill J, LaRusso N . The pathobiology of biliary epithelia. Hepatology. 2002; 35(5):1256-68. DOI: 10.1053/jhep.2002.33541. View

19.

Chen X, Keithly J, Paya C, LaRusso N . Cryptosporidiosis. N Engl J Med. 2002; 346(22):1723-31. DOI: 10.1056/NEJMra013170. View

20.

Dong H, Strome S, Salomao D, Tamura H, Hirano F, Flies D . Tumor-associated B7-H1 promotes T-cell apoptosis: a potential mechanism of immune evasion. Nat Med. 2002; 8(8):793-800. DOI: 10.1038/nm730. View