THE MAMMALIAN CELL-VIRUS RELATIONSHIP : V. SUSCEPTIBILITY AND RESISTANCE OF CELLS IN VITRO TO INFECTION BY COXSACKIE A9 VIRUS

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 2009 Oct 30

PMID 19867177

Citations 2

Authors

L C McLaren

J J Holland

J T SYVERTON

Affiliations

Soon will be listed here.

Abstract

All primary cell cultures tested from numerous human and rhesus monkey tissues were susceptible to Coxsackie A9 virus infection and were found to contain a specific A9 virus receptor. Ability of these cells to adsorb actively virus correlated with the presence of this receptor. Attachment of A9 virus to cell receptor was specifically enhanced by calcium and magnesium ions. Human cells established in continuous culture from pretested susceptible cultures were found to have lost A9 virus susceptibility and A9 receptors but not receptors for adsorption of type 1 poliovirus or B1, B3, B5 Coxsackie viruses. These cultures were able also to produce infectious A9 virus after exposure to viral RNA. Coxsackie A9 virus as well as type 1 poliovirus, inactivated following reaction with debris prepared from susceptible cells, could be recovered fully in infectious form by treatment of the inactive systems at low pH. Recovery of infectious A9 virus was also possible with a chelating compound. Coxsackie A9 virus could also be dissociated by means of low pH after adsorption onto susceptible cells for 2 minutes at 1 degrees C., but not after 1 hour incubation at 37 degrees C., indicating that viral host cell activity may be required in order for irreversible enterovirus attachment to take place.

Citing Articles

The location and nature of enterovirus receptors in susceptible cells.

Holland J, McLaren L J Exp Med. 1961; 114:161-71.

PMID: 13715283 PMC: 2137448. DOI: 10.1084/jem.114.2.161.

The inactivation of enterovirus infectivity by the sulfhydryl reagent p-chloromercuribenzoate.

Choppin P, Philipson L J Exp Med. 1961; 113:713-34.

PMID: 13693271 PMC: 2137380. DOI: 10.1084/jem.113.4.713.

References

Eagle H, HABEL K, Rowe W, HUEBNER R . Viral susceptibility of a human carcinoma cell (strain KB). Proc Soc Exp Biol Med. 1956; 91(3):361-4. DOI: 10.3181/00379727-91-22263. View

Robertson H, BRUNNER K, SYVERTON J . Propagation in vitro of poliomyelitis viruses. VII. pH change of HeLa cell cultures for assay. Proc Soc Exp Biol Med. 1955; 88(1):119-22. DOI: 10.3181/00379727-88-21509. View

Holland J, McLaren L . The mammalian cell-virus relationship. II. Adsorption, reception, and eclipse of poliovirus by HeLa cells. J Exp Med. 1959; 109(5):487-504. PMC: 2136971. DOI: 10.1084/jem.109.5.487. View

Holland J, McLaren L . Improved method for staining cell monolayers for virus plaque counts. J Bacteriol. 1959; 78:596-7. PMC: 290593. DOI: 10.1128/jb.78.4.596-597.1959. View

Eagle H . Propagation in a fluid medium of a human epidermoid carcinoma, strain KB. Proc Soc Exp Biol Med. 1955; 89(3):362-4. DOI: 10.3181/00379727-89-21811. View

Holland J, McLaren L, SYVERTON J . The mammalian cell-virus relationship. IV. Infection of naturally insusceptible cells with enterovirus ribonucleic acid. J Exp Med. 1959; 110(1):65-80. PMC: 2136965. DOI: 10.1084/jem.110.1.65. View

Vogt M, DULBECCO R . Properties of a HeLa cell culture with increased resistance to poliomyelitis virus. Virology. 1958; 5(3):425-34. DOI: 10.1016/0042-6822(58)90037-0. View

CROWELL R, SYVERTON J . The viral range in vitro of a malignant, human epithelial cell (strain HeLa, Gey). IV. The cytopathogenicity of C viruses. J Immunol. 1955; 74(2):169-77. View

Lerner A, Takemoto K . Human amnion cell cultures; susceptibility to viruses and use in primary virus isolations. Proc Soc Exp Biol Med. 1957; 94(1):179-82. DOI: 10.3181/00379727-94-22891. View

10.

PULVERTAFT R, Davies J, WEISS L, Wilkinson J . Studies on tissue cultures of human pathological thyroids. J Pathol Bacteriol. 1959; 77(1):19-32. DOI: 10.1002/path.1700770103. View

11.

Alexander C, KIRBY W, Weinstein H, YOSHIHARA G . Preparation of human amnion tissue cultures. Proc Soc Exp Biol Med. 1956; 92(3):535-8. DOI: 10.3181/00379727-92-22535. View

12.

McLaren L, Holland J, SYVERTON J . The mammalian cell-virus relationship. I. Attachment of poliovirus to cultivated cells of primate and non-primate origin. J Exp Med. 1959; 109(5):475-85. PMC: 2136976. DOI: 10.1084/jem.109.5.475. View

13.

DUNNEBACKE T, ZITCER E . Preparation and cultivation of primary human amnion cells. Cancer Res. 1957; 17(11):1043-6. View

14.

SICKLES G, Mutterer M, FEORINO P, PLAGER H . Recently classified types of Coxsackie virus, group A; behavior in tissue culture. Proc Soc Exp Biol Med. 1955; 90(2):529-31. DOI: 10.3181/00379727-90-22088. View

15.

Hummeler K, KETLER A . Dissociation of poliomyelitis virus from neutralizing antibody. Virology. 1958; 6(1):297-9. DOI: 10.1016/0042-6822(58)90082-5. View

16.

Evans A . Establishment of human adult tonsil cells in continuous culture and their virus susceptibilities. Proc Soc Exp Biol Med. 1957; 96(3):752-7. DOI: 10.3181/00379727-96-23599. View

17.

ZITCER E, DUNNEBACKE T . Transformation of cells from the normal human amnion into established strains. Cancer Res. 1957; 17(11):1047-53. View

18.

Hsiung G . Use of human kidney cultures in the study of enteroviruses. Proc Soc Exp Biol Med. 1959; 102:612-6. DOI: 10.3181/00379727-102-25335. View

19.

Lerner A, Takemoto K, Shelokov A . Human chorion cells: cultivation and susceptibility to viruses. Proc Soc Exp Biol Med. 1957; 95(1):76-80. DOI: 10.3181/00379727-95-23126. View

20.

Mandel B . Studies on the interactions of poliomyelitis virus, antibody, and host cells in tissue culture system. Virology. 1958; 6(2):424-47. DOI: 10.1016/0042-6822(58)90092-8. View