Antioxidant and Cytotoxic Activities of Centella Asiatica (L) Urb

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2009 Oct 30

PMID 19865514

Citations 41

Authors

Frederico Pittella

Rafael C Dutra

Dalton D Junior

Miriam T P Lopes

Nadia R Barbosa

Affiliations

Soon will be listed here.

Abstract

In the present study, the phenolic (Folin-Dennis) and flavonoid (colorimetric assay) constituents, antioxidant [2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) assay] and cytotoxic activities of an aqueous extract (AE) of Centella asiatica leaves were investigated. The aqueous extract (50 g/L) was obtained by infusion followed by cold maceration for 24 h. The levels of phenolic and flavonoid compounds were 2.86 g/100 g and 0.361 g/100 g, respectively. The AE showed elevated DPPH scavenging activity, with an IC(50) value of 31.25 microg/mL. The AE had a promising activity against mouse melanoma (B(16)F(1)), human breast cancer (MDA MB-231) and rat glioma (C(6)) cell lines, with IC(50) values of 698.0, 648.0 and 1000.0 microg/mL, respectively. A positive correlation was established between the level of flavonoids, antioxidant and antitumor activities.

Citing Articles

Optimisation of Ultrasound-Assisted Extraction of Total Phenolics and Flavonoids Content from .

Hiranpradith V, Therdthai N, Soontrunnarudrungsri A, Rungsuriyawiboon O Foods. 2025; 14(2).

PMID: 39856957 PMC: 11765454. DOI: 10.3390/foods14020291.

Evaluating the antifibrotic potential of naringenin, asiatic acid, and icariin using murine and human precision-cut liver slices.

Luo K, Geng Y, Oosterhuis D, de Meijer V, Olinga P Physiol Rep. 2024; 12(21):e16136.

PMID: 39501714 PMC: 11538472. DOI: 10.14814/phy2.16136.

Topical Application of in Wound Healing: Recent Insights into Mechanisms and Clinical Efficacy.

Witkowska K, Paczkowska-Walendowska M, Garbiec E, Cielecka-Piontek J Pharmaceutics. 2024; 16(10).

PMID: 39458583 PMC: 11510310. DOI: 10.3390/pharmaceutics16101252.

Raepenol™ Cream, a Complex of Natural Compounds, Promotes Wound Healing and Relieves Pruritus .

Kim E, Cho N, Park S, Kim H, Yi J, Kim H In Vivo. 2024; 38(5):2318-2327.

PMID: 39187315 PMC: 11363757. DOI: 10.21873/invivo.13697.

Determination of the anticancer activity of standardized extract of (ECa 233) on cell growth and metastatic behavior in oral cancer cells.

Manmuan S, Tubtimsri S, Chaothanaphat N, Issaro N, Tantisira M, Manmuan P Res Pharm Sci. 2024; 19(2):121-147.

PMID: 39035578 PMC: 11257205. DOI: 10.4103/RPS.RPS_81_23.

References

Cao G, Sofic E, Prior R . Antioxidant and prooxidant behavior of flavonoids: structure-activity relationships. Free Radic Biol Med. 1997; 22(5):749-60. DOI: 10.1016/s0891-5849(96)00351-6. View

Kamuhabwa A, Nshimo C, DE Witte P . Cytotoxicity of some medicinal plant extracts used in Tanzanian traditional medicine. J Ethnopharmacol. 2000; 70(2):143-9. DOI: 10.1016/s0378-8741(99)00161-0. View

Xu B, Chang S . A comparative study on phenolic profiles and antioxidant activities of legumes as affected by extraction solvents. J Food Sci. 2007; 72(2):S159-66. DOI: 10.1111/j.1750-3841.2006.00260.x. View

Hsu Y, Kuo P, Lin L, Lin C . Asiatic acid, a triterpene, induces apoptosis and cell cycle arrest through activation of extracellular signal-regulated kinase and p38 mitogen-activated protein kinase pathways in human breast cancer cells. J Pharmacol Exp Ther. 2005; 313(1):333-44. DOI: 10.1124/jpet.104.078808. View

Basile A, Ferrara L, Del Pezzo M, Mele G, Sorbo S, Bassi P . Antibacterial and antioxidant activities of ethanol extract from Paullinia cupana Mart. J Ethnopharmacol. 2005; 102(1):32-6. DOI: 10.1016/j.jep.2005.05.038. View

Hamilton M, Van Remmen H, Drake J, Yang H, Guo Z, Kewitt K . Does oxidative damage to DNA increase with age?. Proc Natl Acad Sci U S A. 2001; 98(18):10469-74. PMC: 56984. DOI: 10.1073/pnas.171202698. View

Sreejayan N, Rao M . Free radical scavenging activity of curcuminoids. Arzneimittelforschung. 1996; 46(2):169-71. View

Park B, Bosire K, Lee E, Lee Y, Kim J . Asiatic acid induces apoptosis in SK-MEL-2 human melanoma cells. Cancer Lett. 2005; 218(1):81-90. DOI: 10.1016/j.canlet.2004.06.039. View

Hyun Yoo H, Park J, Kwon S . In vitro cytotoxic activity of some Korean medicinal plants on human cancer cell lines: enhancement in cytotoxicity by heat processing. Phytother Res. 2007; 21(9):900-3. DOI: 10.1002/ptr.2213. View

10.

Sohal R, Mockett R, Orr W . Mechanisms of aging: an appraisal of the oxidative stress hypothesis. Free Radic Biol Med. 2002; 33(5):575-86. DOI: 10.1016/s0891-5849(02)00886-9. View

11.

Park B, Paek S, Lee Y, Kim S, Lee E, Choi H . Inhibitory effects of asiatic acid on 7,12-dimethylbenz[a]anthracene and 12-O-tetradecanoylphorbol 13-acetate-induced tumor promotion in mice. Biol Pharm Bull. 2007; 30(1):176-9. DOI: 10.1248/bpb.30.176. View

12.

Veerendra Kumar M, Gupta Y . Effect of different extracts of Centella asiatica on cognition and markers of oxidative stress in rats. J Ethnopharmacol. 2002; 79(2):253-60. DOI: 10.1016/s0378-8741(01)00394-4. View

13.

Young I, Woodside J . Antioxidants in health and disease. J Clin Pathol. 2001; 54(3):176-86. PMC: 1731363. DOI: 10.1136/jcp.54.3.176. View

14.

Cho C, Choi D, Cardone M, Kim C, Sinskey A, Rha C . Glioblastoma cell death induced by asiatic acid. Cell Biol Toxicol. 2006; 22(6):393-408. DOI: 10.1007/s10565-006-0104-2. View

15.

Subathra M, Shila S, Devi M, Panneerselvam C . Emerging role of Centella asiatica in improving age-related neurological antioxidant status. Exp Gerontol. 2005; 40(8-9):707-15. DOI: 10.1016/j.exger.2005.06.001. View

16.

Gurfinkel D, Chow S, Hurren R, Gronda M, Henderson C, Berube C . Disruption of the endoplasmic reticulum and increases in cytoplasmic calcium are early events in cell death induced by the natural triterpenoid Asiatic acid. Apoptosis. 2006; 11(9):1463-71. DOI: 10.1007/s10495-006-9086-z. View

17.

Stewart D . Mechanisms of resistance to cisplatin and carboplatin. Crit Rev Oncol Hematol. 2007; 63(1):12-31. DOI: 10.1016/j.critrevonc.2007.02.001. View

18.

Barbosa N, Pittella F, Gattaz W . Centella asiatica water extract inhibits iPLA2 and cPLA2 activities in rat cerebellum. Phytomedicine. 2008; 15(10):896-900. DOI: 10.1016/j.phymed.2008.02.007. View

19.

Fiore G, Nencini C, Cavallo F, Capasso A, Bader A, Giorgi G . In vitro antiproliferative effect of six Salvia species on human tumor cell lines. Phytother Res. 2006; 20(8):701-3. DOI: 10.1002/ptr.1911. View

20.

Mensor L, Menezes F, Leitao G, Reis A, Dos Santos T, Coube C . Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother Res. 2001; 15(2):127-30. DOI: 10.1002/ptr.687. View