Optimization of the Matrix Solid Phase Dispersion Extraction Procedure for the Analysis of Polybrominated Diphenyl Ethers in Human Placenta

Overview

Journal Anal Chem

Specialty Chemistry

Date 2009 Oct 30

PMID 19863067

Citations 12

Authors

R M A Priyanthi S Dassanayake

Hua Wei

Rachel C Chen

An Li

Affiliations

Soon will be listed here.

Abstract

Characterization of prenatal exposure to hazardous chemicals most often relies upon the analysis of cord blood. However, human placenta is an appropriate tissue alternative with noteworthy advantages. Owing to analytical challenges, reports on placental levels of toxic chemicals are limited. The purpose of this study was to establish a reliable, cost-effective, and relatively fast and simple method to extract polybrominated diphenyl ethers (PBDEs) from human placenta for analysis using gas chromatography coupled with mass spectrometry (GC/MS). The matrix solid phase dispersion (MSPD) method was optimized for the extraction and analysis of 43 PBDEs (including BDE209) from human placenta samples. Different sorbents, sample conditions, grinding methods, elution solvents, and single and repeated extractions were compared for their effects on the extraction efficiency. The performance of the optimized method was validated by analyzing spiked placenta samples and a standard reference material of fish tissue. Congener specific PBDE recovery ranged from 91% to 114% for the spiked samples and 89% to 115% for a standard reference material (SRM) of fish tissue. The optimized MSPD procedure was compared with two conventional extraction methods. The extraction efficiency of MSPD was found to be comparable with that of the traditional Soxhlet method and superior to that using a liquid extraction method. Twenty two PBDEs were detected in all of the five samples collected in Chicago in 2008. This is the first description of PBDEs detected in human placentas in the U.S.

Citing Articles

Brominated flame retardants and legacy organochlorines in archived human placenta samples: Sex differences, temporal analysis and associations with infant birth weight.

Ruis M, Hoffman K, Stapleton H Chemosphere. 2023; 322:138170.

PMID: 36804493 PMC: 10039416. DOI: 10.1016/j.chemosphere.2023.138170.

Levels and distribution of polybrominated diphenyl ethers in humans and environmental compartments: a comprehensive review of the last five years of research.

Klincic D, Dvorscak M, Jagic K, Mendas G, Herceg Romanic S Environ Sci Pollut Res Int. 2020; 27(6):5744-5758.

PMID: 31933075 DOI: 10.1007/s11356-020-07598-7.

A preliminary study on prenatal polybrominated diphenyl ether serum concentrations and intrinsic functional network organization and executive functioning in childhood.

de Water E, Curtin P, Zilverstand A, Sjodin A, Bonilla A, Herbstman J J Child Psychol Psychiatry. 2019; 60(9):1010-1020.

PMID: 30882909 PMC: 7105394. DOI: 10.1111/jcpp.13040.

PBDE-47 and PBDE mixture (DE-71) toxicities and liver transcriptomic changes at PND 22 after in utero/postnatal exposure in the rat.

Dunnick J, Shockley K, Pandiri A, Kissling G, Gerrish K, Ton T Arch Toxicol. 2018; 92(11):3415-3433.

PMID: 30206662 PMC: 6706773. DOI: 10.1007/s00204-018-2292-y.

Temporal trends and developmental patterns of plasma polybrominated diphenyl ether concentrations over a 15-year period between 1998 and 2013.

Cowell W, Sjodin A, Jones R, Wang Y, Wang S, Herbstman J J Expo Sci Environ Epidemiol. 2018; 29(1):49-60.

PMID: 29618764 PMC: 6429949. DOI: 10.1038/s41370-018-0031-3.

References

Schantz S . Developmental neurotoxicity of PCBs in humans: what do we know and where do we go from here?. Neurotoxicol Teratol. 1996; 18(3):217-27; discussion 229-76. DOI: 10.1016/s0892-0362(96)90001-x. View

BLIGH E, Dyer W . A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959; 37(8):911-7. DOI: 10.1139/o59-099. View

Main K, Kiviranta H, Virtanen H, Sundqvist E, Tuomisto J, Tuomisto J . Flame retardants in placenta and breast milk and cryptorchidism in newborn boys. Environ Health Perspect. 2007; 115(10):1519-26. PMC: 2022640. DOI: 10.1289/ehp.9924. View

Myllynen P, Pasanen M, Pelkonen O . Human placenta: a human organ for developmental toxicology research and biomonitoring. Placenta. 2005; 26(5):361-71. DOI: 10.1016/j.placenta.2004.09.006. View

Valsamaki V, Boti V, Sakkas V, Albanis T . Determination of organochlorine pesticides and polychlorinated biphenyls in chicken eggs by matrix solid phase dispersion. Anal Chim Acta. 2007; 573-574:195-201. DOI: 10.1016/j.aca.2006.03.050. View

Barker S . Matrix solid-phase dispersion. J Chromatogr A. 2000; 885(1-2):115-27. DOI: 10.1016/s0021-9673(00)00249-1. View

Wei H, Dassanayake P, Li A . Parametric evaluation for programmable temperature vaporization large volume injection in gas chromatographic determination of polybrominated diphenyl ethers. Int J Environ Anal Chem. 2010; 90(7):535-547. PMC: 2850545. DOI: 10.1080/03067310902871299. View

Chao H, Wang S, Lee W, Wang Y, Papke O . Levels of polybrominated diphenyl ethers (PBDEs) in breast milk from central Taiwan and their relation to infant birth outcome and maternal menstruation effects. Environ Int. 2006; 33(2):239-45. DOI: 10.1016/j.envint.2006.09.013. View

Doucet J, Tague B, Arnold D, Cooke G, Hayward S, Goodyer C . Persistent organic pollutant residues in human fetal liver and placenta from Greater Montreal, Quebec: a longitudinal study from 1998 through 2006. Environ Health Perspect. 2009; 117(4):605-10. PMC: 2679605. DOI: 10.1289/ehp.0800205. View

10.

Morzycka B . Simple method for the determination of trace levels of pesticides in honeybees using matrix solid-phase dispersion and gas chromatography. J Chromatogr A. 2002; 982(2):267-73. DOI: 10.1016/s0021-9673(02)01505-4. View

11.

Saito K, Sjodin A, Sandau C, Davis M, Nakazawa H, Matsuki Y . Development of a accelerated solvent extraction and gel permeation chromatography analytical method for measuring persistent organohalogen compounds in adipose and organ tissue analysis. Chemosphere. 2004; 57(5):373-81. DOI: 10.1016/j.chemosphere.2004.04.050. View

12.

Barker S . Matrix solid phase dispersion (MSPD). J Biochem Biophys Methods. 2006; 70(2):151-62. DOI: 10.1016/j.jbbm.2006.06.005. View

13.

Martinez A, Ramil M, Montes R, Hernanz D, Rubi E, Rodriguez I . Development of a matrix solid-phase dispersion method for the screening of polybrominated diphenyl ethers and polychlorinated biphenyls in biota samples using gas chromatography with electron-capture detection. J Chromatogr A. 2005; 1072(1):83-91. DOI: 10.1016/j.chroma.2004.12.034. View

14.

Albero B, Sanchez-Brunete C, Tadeo J . Determination of organophosphorus pesticides in fruit juices by matrix solid-phase dispersion and gas chromatography. J Agric Food Chem. 2003; 51(24):6915-21. DOI: 10.1021/jf030414m. View

15.

Lino C, Azzolini C, Nunes D, Silva J, DA Silveira M . Methods for the determination of organochlorine pesticide residues in human serum. J Chromatogr B Biomed Sci Appl. 1998; 716(1-2):147-52. DOI: 10.1016/s0378-4347(98)00284-9. View

16.

Norlock F, Jang J, Zou Q, Schoonover T, Li A . Large-volume injection PTV-GC-MS analysis of polycyclic aromatic hydrocarbons in air and sediment samples. J Air Waste Manag Assoc. 2004; 52(1):19-26. DOI: 10.1080/10473289.2002.10470752. View

17.

Myren M, Mose T, Mathiesen L, Knudsen L . The human placenta--an alternative for studying foetal exposure. Toxicol In Vitro. 2007; 21(7):1332-40. DOI: 10.1016/j.tiv.2007.05.011. View

18.

Gomara B, Herrero L, Ramos J, Mateo J, Fernandez M, Garcia J . Distribution of polybrominated diphenyl ethers in human umbilical cord serum, paternal serum, maternal serum, placentas, and breast milk from Madrid population, Spain. Environ Sci Technol. 2007; 41(20):6961-8. DOI: 10.1021/es0714484. View

19.

Gomara B, Herrero L, Bordajandi L, Gonzalez M . Quantitative analysis of polybrominated diphenyl ethers in adipose tissue, human serum and foodstuff samples by gas chromatography with ion trap tandem mass spectrometry and isotope dilution. Rapid Commun Mass Spectrom. 2005; 20(2):69-74. DOI: 10.1002/rcm.2264. View

20.

Li Z, Zhang Z, Zhou Q, Wang Q, Gao R, Wang Q . Stereo- and enantioselective determination of pesticides in soil by using achiral and chiral liquid chromatography in combination with matrix solid-phase dispersion. J AOAC Int. 2003; 86(3):521-8. View