Impact of the Size of Zona Pellucida Thinning Area on Vitrified-warmed Cleavage-stage Embryo Transfers: a Prospective, Randomized Study

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2009 Oct 16

PMID 19830543

Citations 17

Authors

Kenichiro Hiraoka

Kaori Hiraoka

Toshitaka Horiuchi

Tomoyo Kusuda

Shinichiro Okano

Masayuki Kinutani

Kazuo Kinutani

Affiliations

Soon will be listed here.

Abstract

Purpose: The aim of this study was to determine if the size of zona pellucida thinning area by laser assisted hatching could affect the rates of pregnancy and implantation for vitrified-warmed embryo transfers at the cleavage-stage.

Methods: A total of 120 vitrified-warmed cleavage-stage embryo transfers were randomly assigned to either quarter or half of zona pellucida thinning group.

Results: The rates of clinical pregnancy (46.7 versus 25.0%) and implantation (32.0 versus 16.2%) were significantly greater in the half thinning group than in the quarter thinning group (P = 0.0218 and P = 0.0090, respectively).

Conclusions: The results of this investigation show that, in vitrified-warmed embryo transfers at the cleavage-stage, the size of zona pellucida thinning area by laser assisted hatching impacts the rate of clinical pregnancy and implantation and that half of zona pellucida thinning significantly increases both of these results compared with quarter of zona pellucida thinning.

Citing Articles

Clinical and neonatal outcomes of complete zona pellucida removal by laser-assisted hatching after single vitrified-warmed blastocyst transfer.

Baatarsuren M, Jamiyansuren J, Ganbaatar C, Sengebaljir D, Erdenekhuyag B, Enkhbaatar S Lasers Med Sci. 2024; 39(1):51.

PMID: 38285329 DOI: 10.1007/s10103-024-04002-5.

Assisted Hatching Treatment of Piezo-Mediated Small Hole on Zona Pellucida in Morula Stage Embryos Improves Embryo Implantation and Litter Size in Mice.

Hao X, Zhao Y, Ding K, Xue F, Wang X, Yang Q Front Cell Dev Biol. 2021; 9:746104.

PMID: 34746141 PMC: 8564066. DOI: 10.3389/fcell.2021.746104.

The effect of chemical treatment of the sheep embryo zona pellucida on the ability of blastocysts to hatch after vitrification and warming.

Mousavi F, Ahmadi E, Shirazi A, Shams-Esfandabadi N, Nazari H Vet Med Sci. 2021; 8(1):405-410.

PMID: 34532986 PMC: 8788952. DOI: 10.1002/vms3.632.

Assisted hatching on assisted conception (in vitro fertilisation (IVF) and intracytoplasmic sperm injection (ICSI)).

Lacey L, Hassan S, Franik S, Seif M, Akhtar M Cochrane Database Syst Rev. 2021; 3:CD001894.

PMID: 33730422 PMC: 8094760. DOI: 10.1002/14651858.CD001894.pub6.

Failure of complete hatching of ICSI-derived human blastocyst by cell herniation via small slit and insufficient expansion despite ongoing cell proliferation.

Inoue T, Uemura M, Miyazaki K, Yamashita Y J Assist Reprod Genet. 2019; 36(8):1579-1589.

PMID: 31321595 PMC: 6708026. DOI: 10.1007/s10815-019-01521-x.

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