Assessment of Sex Chromosomes of Human Embryos Arising from Monopronucleus Zygotes in in Vitro Fertilization and Intracytoplasmic Sperm Injection Cycles of Chinese Women

Overview

Journal Gynecol Obstet Invest

Specialty Gynecology & Obstetrics

Date 2009 Oct 16

PMID 19829001

Citations 6

Authors

Junhao Yan

Yuan Li

Yuhua Shi

Huai L Feng

Shanshan Gao

Zi-Jiang Chen

Affiliations

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Abstract

Objectives: To examine abnormal fertilization phenomena in blastomeres of embryos generated from conventional IVF and ICSI displaying a single pronucleus at the zygote stage.

Methods: 132 embryos from monopronuclear zygotes (1PN) generated from conventional IVF and ICSI were examined by FISH (fluorescent in situ hybridization) with X, Y dual color centromere probes.

Results: In the embryos that were obtained from conventional IVF, the percentage of diploid, monoploid and mosaic sex chromosome were 54.35, 23.91 and 21.74%, respectively. In the embryos from 1PN zygotes derived from ICSI, the percentage of diploid, monoploid and mosaic sex chromosomes were 31.51, 31.51 and 36.99%, respectively. For monoploid embryos, the ratio of XO was significantly higher than that of YO in both conventional IVF and ICSI groups.

Conclusion: The results demonstrated that the majority of embryos derived from 1PN zygotes generated with conventional IVF or ICSI have a high incidence of aneuploidy. Furthermore, the sex chromosome diploid ratio of embryos from 1PN zygotes derived from conventional IVF was significantly higher than those generated from ICSI procedure in Chinese women. Nevertheless, the high proportion of aneuploid embryos suggests that such embryos should not be utilized for embryo transfer.

Citing Articles

The aneuploidy testing of blastocysts developing from 0PN and 1PN zygotes in conventional IVF through TE-biopsy PGT-A and minimally invasive PGT-A.

Zhao H, Yuan P, Chen X, Lin H, Zhao J, Huang J Front Reprod Health. 2022; 4:966909.

PMID: 36303642 PMC: 9580634. DOI: 10.3389/frph.2022.966909.

Clinical outcomes of transfer of frozen and thawed single blastocysts derived from nonpronuclear and monopronuclear zygotes.

Hondo S, Arichi A, Muramatsu H, Omura N, Ito K, Komine H Reprod Med Biol. 2019; 18(3):278-283.

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Assessment of developmental potential of human single pronucleated zygotes derived from conventional in vitro fertilization.

Kai Y, Moriwaki H, Yumoto K, Iwata K, Mio Y J Assist Reprod Genet. 2018; 35(8):1377-1384.

PMID: 29959619 PMC: 6086793. DOI: 10.1007/s10815-018-1241-2.

Correlation between the pronucleus size and the potential for human single pronucleus zygotes to develop into blastocysts : 1PN zygotes with large pronuclei can expect an embryo development to the blastocyst stage that is similar to the development....

Araki E, Itoi F, Honnma H, Asano Y, Oguri H, Nishikawa K J Assist Reprod Genet. 2018; 35(5):817-823.

PMID: 29479641 PMC: 5984884. DOI: 10.1007/s10815-018-1137-1.

Birth of nine normal healthy babies following transfer of blastocysts derived from human single-pronucleate zygotes.

Itoi F, Asano Y, Shimizu M, Honnma H, Murata Y J Assist Reprod Genet. 2015; 32(9):1401-7.

PMID: 26111861 PMC: 4595390. DOI: 10.1007/s10815-015-0518-y.