Mechanisms of NKT Cell Anergy Induction Involve Cbl-b-promoted Monoubiquitination of CARMA1

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2009 Oct 10

PMID 19815501

Citations 43

Authors

Satoshi Kojo

Chris Elly

Yohsuke Harada

Wallace Y Langdon

Mitchell Kronenberg

Yun-Cai Liu

Affiliations

Soon will be listed here.

Abstract

Repeated injection of alpha-galactosylceramide, an agonistic ligand for natural killer T (NKT) cells, results in long-term unresponsiveness or anergy, which severely limits its clinical application. However, the molecular mechanisms leading to NKT anergy induction remain unclear. We show here that the decreased IFN-gamma production and failed tumor rejection observed in anergized NKT cells are rescued by Cbl-b deficiency. Cbl-b E3 ligase activity is critical for the anergy induction, as revealed by the similarity between Cbl-b(-/-) and its RING finger mutant NKT cells. Cbl-b binds and promotes monoubiquitination to CARMA1, a critical signaling molecule in NFkappaB activation. Ubiquitin conjugation to CARMA1 disrupts its complex formation with Bcl10 without affecting its protein stability. In addition, CARMA1(-/-) NKT cells are defective in IFN-gamma production. The study identifies an important signaling pathway linking Cbl-b-induced monoubiquitination to NFkappaB activation in NKT cell anergy induction, which may help design approaches for human cancer therapy.

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