Toward a Durable Anti-HIV Gene Therapy Based on RNA Interference

Overview

Journal Ann N Y Acad Sci

Specialty Science

Date 2009 Oct 3

PMID 19796072

Citations 11

Authors

Ben Berkhout

Affiliations

Soon will be listed here.

Abstract

Basic research in the field of molecular biology led to the discovery of the mechanism of RNA interference (RNAi) in Caenorhabditis elegans in 1998. RNAi is now widely appreciated as an important gene control mechanism in mammals, and several RNAi-based gene-silencing applications have already been used in clinical trials. In this review I will discuss RNAi approaches to inhibit the pathogenic human immunodeficiency virus type 1 (HIV-1), which establishes a chronic infection that would most likely require a durable gene therapy approach. Viruses, such as HIV-1, are particularly difficult targets for RNAi attack because they mutate frequently, which allows viral escape by mutation of the RNAi target sequence. Combinatorial RNAi strategies are required to prevent viral escape.

Citing Articles

Inhibition of SARS-CoV-2 Replication by a Small Interfering RNA Targeting the Leader Sequence.

Tolksdorf B, Nie C, Niemeyer D, Rohrs V, Berg J, Lauster D Viruses. 2021; 13(10).

PMID: 34696460 PMC: 8539227. DOI: 10.3390/v13102030.

CRISPR-Cas13a Inhibits HIV-1 Infection.

Yin L, Zhao F, Sun H, Wang Z, Huang Y, Zhu W Mol Ther Nucleic Acids. 2020; 21:147-155.

PMID: 32585623 PMC: 7321785. DOI: 10.1016/j.omtn.2020.05.030.

Influence of a 3' Terminal Ribozyme on AgoshRNA Biogenesis and Activity.

Herrera-Carrillo E, Gao Z, Berkhout B Mol Ther Nucleic Acids. 2019; 16:452-462.

PMID: 31048184 PMC: 6488825. DOI: 10.1016/j.omtn.2019.04.001.

Influence of the loop size and nucleotide composition on AgoshRNA biogenesis and activity.

Herrera-Carrillo E, Harwig A, Berkhout B RNA Biol. 2017; 14(11):1559-1569.

PMID: 28569591 PMC: 5785215. DOI: 10.1080/15476286.2017.1328349.

Silencing of HIV-1 by AgoshRNA molecules.

Herrera-Carrillo E, Harwig A, Berkhout B Gene Ther. 2017; 24(8):453-461.

PMID: 28553929 DOI: 10.1038/gt.2017.44.

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