Heterogeneity of Non-cycling and Cycling Synchronized Murine Hematopoietic Stem/progenitor Cells

Overview

Journal J Cell Physiol

Specialties Cell Biology
Physiology

Date 2009 Sep 24

PMID 19774557

Citations 11

Authors

Gerald A Colvin

David Berz

Liansheng Liu

Mark S Dooner

Gerri Dooner

Sheila Pascual

Samuel Chung

Yunxia Sui

Peter J Quesenberry

Affiliations

Soon will be listed here.

Abstract

Purified long-term multilineage repopulating marrow stem cells have been considered to be homogenous, but functionally these cells are heterogeneous. Many investigators urge clonal studies to define stem cells but, if stem cells are truly heterogeneous, clonal studies can only define heterogeneity. We have determined the colony growth and differentiation of individual lineage negative, rhodamine low, Hoechst low (LRH) stem cells at various times in cytokine culture, corresponding to specific cell cycle stages. These highly purified and cycle synchronized (98% in S phase at 40 h of culture) stem cells were exposed to two cytokine cocktails for 0, 18, 32, or 40 h and clonal differentiation assessed 14 days later. Total heterogeneity as to gross colony morphology and differentiation stage was demonstrated. This heterogeneity showed patterns of differentiation at different cycle times. These data hearken to previous suggestions that stem cells might be similar to radioactive isotopes; decay rate of a population of radioisotopes being highly predictable, while the decay of individual nuclei is heterogeneous and unpredictable (Till et al., 1964). Marrow stem cells may be most adequately defined on a population basis; stem cells existing in a continuum of reversible change rather than a hierarchy.

Citing Articles

Multipotent/pluripotent stem cell populations in stromal tissues and peripheral blood: exploring diversity, potential, and therapeutic applications.

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The Essence of Quiescence.

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The universal stem cell.

Quesenberry P, Wen S, Goldberg L, Dooner M Leukemia. 2022; 36(12):2784-2792.

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Hematopoietic Stem Cells: Uncomfortable Considerations.

Quesenberry P, Goldberg L, Dooner M, Wen S Curr Mol Biol Rep. 2021; 3(3):165-171.

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Heuristic bias in stem cell biology.

Quesenberry P, Borgovan T, Nwizu C, Dooner M, Goldberg L Stem Cell Res Ther. 2019; 10(1):241.

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