Spin Labeling EPR

Overview

Journal Photosynth Res

Publisher Springer

Date 2009 Sep 4

PMID 19728138

Citations 65

Authors

Johann P Klare

Heinz-Jurgen Steinhoff

Affiliations

Soon will be listed here.

Abstract

Site-directed spin labeling in combination with electron paramagnetic resonance spectroscopy has emerged as an efficient tool to elucidate the structure and conformational dynamics of biomolecules under native-like conditions. This article summarizes the basics as well as recent progress of site-directed spin labeling. Continuous wave EPR spectra analyses and pulse EPR techniques are reviewed with special emphasis on applications to the sensory rhodopsin-transducer complex mediating the photophobic response of the halophilic archaeum Natronomonas pharaonis and the photosynthetic reaction center from Rhodobacter sphaeroides R26.

Citing Articles

Structural Flexibility of Tau in Its Interaction with Microtubules as Viewed by Site-Directed Spin Labeling EPR Spectroscopy.

Martinho M, Allegro D, Etienne E, Lohberger C, Bonucci A, Belle V Methods Mol Biol. 2024; 2754:55-75.

PMID: 38512660 DOI: 10.1007/978-1-0716-3629-9_3.

Integrative dynamic structural biology unveils conformers essential for the oligomerization of a large GTPase.

Peulen T, Hengstenberg C, Biehl R, Dimura M, Lorenz C, Valeri A Elife. 2023; 12.

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UNILIPID, a Methodology for Energetically Accurate Prediction of Protein Insertion into Implicit Membranes of Arbitrary Shape.

Lanrezac A, Baaden M Membranes (Basel). 2023; 13(3).

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Illuminating Intrinsically Disordered Proteins with Integrative Structural Biology.

Evans R, Ramisetty S, Kulkarni P, Weninger K Biomolecules. 2023; 13(1).

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