Real-time DNA Microarray Analysis

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2009 Sep 3

PMID 19723688

Citations 13

Authors

Arjang Hassibi

Haris Vikalo

Jose Luis Riechmann

Babak Hassibi

Affiliations

Soon will be listed here.

Abstract

We present a quantification method for affinity-based DNA microarrays which is based on the real-time measurements of hybridization kinetics. This method, i.e. real-time DNA microarrays, enhances the detection dynamic range of conventional systems by being impervious to probe saturation in the capturing spots, washing artifacts, microarray spot-to-spot variations, and other signal amplitude-affecting non-idealities. We demonstrate in both theory and practice that the time-constant of target capturing in microarrays, similar to all affinity-based biosensors, is inversely proportional to the concentration of the target analyte, which we subsequently use as the fundamental parameter to estimate the concentration of the analytes. Furthermore, to empirically validate the capabilities of this method in practical applications, we present a FRET-based assay which enables the real-time detection in gene expression DNA microarrays.

Citing Articles

Inherent stochasticity, noise and limits of detection in continuous and time-gated fluorescence systems.

Vitale N, Hassibi A, Soh H, Murmann B, Lee T PLoS One. 2024; 19(12):e0313949.

PMID: 39715245 PMC: 11666023. DOI: 10.1371/journal.pone.0313949.

Surface-modified CMOS biosensors.

Dehghandehnavi F, Sajal M, Dandin M Front Bioeng Biotechnol. 2024; 12:1441430.

PMID: 39569164 PMC: 11576298. DOI: 10.3389/fbioe.2024.1441430.

The Intestinal Microbiota May Be a Potential Theranostic Tool for Personalized Medicine.

Di Domenico M, Ballini A, Boccellino M, Scacco S, Lovero R, Charitos I J Pers Med. 2022; 12(4).

PMID: 35455639 PMC: 9024566. DOI: 10.3390/jpm12040523.

An array-based melt curve analysis method for the identification and classification of closely related pathogen strains.

Hassibi A, Ebert J, Bolouki S, Anemogiannis A, Mazarei G, Li Y Biol Methods Protoc. 2020; 3(1):bpy005.

PMID: 32161799 PMC: 6994036. DOI: 10.1093/biomethods/bpy005.

A Fully Integrated CMOS Fluorescence Biochip for DNA and RNA Testing.

Manickam A, Singh R, McDermott M, Wood N, Bolouki S, Naraghi-Arani P IEEE J Solid-State Circuits. 2019; 52(11):2857-2870.

PMID: 30853715 PMC: 6407865. DOI: 10.1109/JSSC.2017.2754363.

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