Desmocollin 3-mediated Binding is Crucial for Keratinocyte Cohesion and is Impaired in Pemphigus

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2009 Sep 1

PMID 19717567

Citations 48

Authors

Volker Spindler

Wolfgang-Moritz Heupel

Athina Efthymiadis

Enno Schmidt

Rudiger Eming

Christian Rankl

Peter Hinterdorfer

Thomas Muller

Detlev Drenckhahn

Jens Waschke

Affiliations

Soon will be listed here.

Abstract

Desmocollin (Dsc) 1-3 and desmoglein (Dsg) 1-4, transmembrane proteins of the cadherin family, form the adhesive core of desmosomes. Here we provide evidence that Dsc3 homo- and heterophilic trans-interaction is crucial for epidermal integrity. Single molecule atomic force microscopy (AFM) revealed homophilic trans-interaction of Dsc3. Dsc3 displayed heterophilic interaction with Dsg1 but not with Dsg3. A monoclonal antibody targeted against the extracellular domain reduced homophilic and heterophilic binding as measured by AFM, caused intraepidermal blistering in a model of human skin, and a loss of intercellular adhesion in cultured keratinocytes. Because autoantibodies against Dsg1 are associated with skin blistering in pemphigus, we characterized the role of Dsc3 binding for pemphigus pathogenesis. In contrast to AFM experiments, laser tweezer trapping revealed that pemphigus autoantibodies reduced binding of Dsc3-coated beads to the keratinocyte cell surface. These data indicate that loss of heterophilic Dsc3/Dsg1 binding may contribute to pemphigus skin blistering.

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