Single-molecule Analysis Reveals That the Lagging Strand Increases Replisome Processivity but Slows Replication Fork Progression

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2009 Aug 12

PMID 19666586

Citations 57

Authors

Nina Y Yao

Roxana E Georgescu

Jeff Finkelstein

Michael E ODonnell

Affiliations

Soon will be listed here.

Abstract

Single-molecule techniques are developed to examine mechanistic features of individual E. coli replisomes during synthesis of long DNA molecules. We find that single replisomes exhibit constant rates of fork movement, but the rates of different replisomes vary over a surprisingly wide range. Interestingly, lagging strand synthesis decreases the rate of the leading strand, suggesting that lagging strand operations exert a drag on replication fork progression. The opposite is true for processivity. The lagging strand significantly increases the processivity of the replisome, possibly reflecting the increased grip to DNA provided by 2 DNA polymerases anchored to sliding clamps on both the leading and lagging strands.

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