Adhesion, Spreading and Osteogenic Differentiation of Mesenchymal Stem Cells Cultured on Micropatterned Amorphous Diamond, Titanium, Tantalum and Chromium Coatings on Silicon

Overview

Journal J Mater Sci Mater Med

Publisher Springer

Specialty Biomedical Engineering

Date 2009 Aug 6

PMID 19655235

Citations 12

Authors

Sami Myllymaa

Emilia Kaivosoja

Katja Myllymaa

Tarvo Sillat

Hannu Korhonen

Reijo Lappalainen

Yrjo T Konttinen

Affiliations

Soon will be listed here.

Abstract

It was hypothesized that human mesenchymal stromal cell (hMSC) can be guided by patterned and plain amorphous diamond (AD), titanium (Ti), tantalum (Ta) and chromium (Cr) coatings, produced on silicon wafer using physical vapour deposition and photolithography. At 7.5 h hMSCs density was 3.0-3.5 x higher (P < 0.0003, except Ti) and cells were smaller (68 vs. 102 microm, P 0.000006-0.02) on patterns than on silicon background. HMSC-covered surface of the background silicon was lower on Ti than AD patterns (P = 0.015), but at 5 days this had reversed (P = 0.006). At 7.5 h focal vinculin adhesions and actin cytoskeleton were outgoing from pattern edges so cells assumed geometric square shapes. Patterns allowed induced osteogenesis, but less effectively than plain surfaces, except for AD, which could be used to avoid osseointegration. All these biomaterial patterns exert direct early, intermediate and late guidance on hMSCs and osteogenic differentiation, but indirect interactions exist with cells on silicon background.

Citing Articles

Effect of Hydrothermal Media on the in-situ Whisker Growth on Biphasic Calcium Phosphate Ceramics.

Feng C, Wu Y, Cao Q, Li X, Zhu X, Zhang X Int J Nanomedicine. 2021; 16:147-159.

PMID: 33456309 PMC: 7804068. DOI: 10.2147/IJN.S280130.

Fabrication of Micro- and Nanopillars from Pyrolytic Carbon and Tetrahedral Amorphous Carbon.

Heikkinen J, Peltola E, Wester N, Koskinen J, Laurila T, Franssila S Micromachines (Basel). 2019; 10(8).

PMID: 31370267 PMC: 6723446. DOI: 10.3390/mi10080510.

Engineering microdent structures of bone implant surfaces to enhance osteogenic activity in MSCs.

Li S, Chow T, Chu J Biochem Biophys Rep. 2017; 9:100-105.

PMID: 29114582 PMC: 5632706. DOI: 10.1016/j.bbrep.2016.11.016.

Behavior of Human Bone Marrow-Derived Mesenchymal Stem Cells on Various Titanium-Based Coatings.

Qu C, Kaitainen S, Kroger H, Lappalainen R, Lammi M Materials (Basel). 2017; 9(10).

PMID: 28773947 PMC: 5456604. DOI: 10.3390/ma9100827.

Semi-quantitative monitoring of confluence of adherent mesenchymal stromal cells on calcium-phosphate granules by using widefield microscopy images.

Piccinini F, Pierini M, Lucarelli E, Bevilacqua A J Mater Sci Mater Med. 2014; 25(10):2395-410.

PMID: 24863020 DOI: 10.1007/s10856-014-5242-0.

References

Ito Y . Surface micropatterning to regulate cell functions. Biomaterials. 1999; 20(23-24):2333-42. DOI: 10.1016/s0142-9612(99)00162-3. View

Lehto V, Virtanen I . Vinculin in cultured bovine lens-forming cells. Cell Differ. 1985; 16(3):153-60. DOI: 10.1016/0045-6039(85)90512-3. View

Wagner W, Horn P, Castoldi M, Diehlmann A, Bork S, Saffrich R . Replicative senescence of mesenchymal stem cells: a continuous and organized process. PLoS One. 2008; 3(5):e2213. PMC: 2374903. DOI: 10.1371/journal.pone.0002213. View

Pirone D, Qi L, Colecraft H, Chen C . Spatial patterning of gene expression using surface-immobilized recombinant adenovirus. Biomed Microdevices. 2008; 10(4):561-6. DOI: 10.1007/s10544-008-9166-7. View

Dalby M, Riehle M, Johnstone H, Affrossman S, Curtis A . Investigating the limits of filopodial sensing: a brief report using SEM to image the interaction between 10 nm high nano-topography and fibroblast filopodia. Cell Biol Int. 2004; 28(3):229-36. DOI: 10.1016/j.cellbi.2003.12.004. View

Sjostrom T, Dalby M, Hart A, Tare R, Oreffo R, Su B . Fabrication of pillar-like titania nanostructures on titanium and their interactions with human skeletal stem cells. Acta Biomater. 2009; 5(5):1433-41. DOI: 10.1016/j.actbio.2009.01.007. View

Levon J, Myllymaa K, Kouri V, Rautemaa R, Kinnari T, Myllymaa S . Patterned macroarray plates in comparison of bacterial adhesion inhibition of tantalum, titanium, and chromium compared with diamond-like carbon. J Biomed Mater Res A. 2009; 92(4):1606-13. DOI: 10.1002/jbm.a.32486. View

Oliveira A, Malafaya P, Reis R . Sodium silicate gel as a precursor for the in vitro nucleation and growth of a bone-like apatite coating in compact and porous polymeric structures. Biomaterials. 2003; 24(15):2575-84. DOI: 10.1016/s0142-9612(03)00060-7. View

Chaubey A, Ross K, Leadbetter R, Burg K . Surface patterning: tool to modulate stem cell differentiation in an adipose system. J Biomed Mater Res B Appl Biomater. 2007; 84(1):70-8. DOI: 10.1002/jbm.b.30846. View

10.

Dalby M, McCloy D, Robertson M, Wilkinson C, Oreffo R . Osteoprogenitor response to defined topographies with nanoscale depths. Biomaterials. 2005; 27(8):1306-15. DOI: 10.1016/j.biomaterials.2005.08.028. View

11.

Brock A, Chang E, Ho C, LeDuc P, Jiang X, Whitesides G . Geometric determinants of directional cell motility revealed using microcontact printing. Langmuir. 2003; 19(5):1611-7. DOI: 10.1021/la026394k. View

12.

Kunzler T, Huwiler C, Drobek T, Voros J, Spencer N . Systematic study of osteoblast response to nanotopography by means of nanoparticle-density gradients. Biomaterials. 2007; 28(33):5000-6. DOI: 10.1016/j.biomaterials.2007.08.009. View

13.

Berry C, Curtis A, Oreffo R, Agheli H, Sutherland D . Human fibroblast and human bone marrow cell response to lithographically nanopatterned adhesive domains on protein rejecting substrates. IEEE Trans Nanobioscience. 2007; 6(3):201-9. DOI: 10.1109/tnb.2007.903457. View

14.

Stiehler M, Lind M, Mygind T, Baatrup A, Dolatshahi-Pirouz A, Li H . Morphology, proliferation, and osteogenic differentiation of mesenchymal stem cells cultured on titanium, tantalum, and chromium surfaces. J Biomed Mater Res A. 2007; 86(2):448-58. DOI: 10.1002/jbm.a.31602. View

15.

Eriksson C, Nygren H, Ohlson K . Implantation of hydrophilic and hydrophobic titanium discs in rat tibia: cellular reactions on the surfaces during the first 3 weeks in bone. Biomaterials. 2004; 25(19):4759-66. DOI: 10.1016/j.biomaterials.2003.12.006. View

16.

Flemming R, Murphy C, Abrams G, Goodman S, Nealey P . Effects of synthetic micro- and nano-structured surfaces on cell behavior. Biomaterials. 1999; 20(6):573-88. DOI: 10.1016/s0142-9612(98)00209-9. View

17.

Mwenifumbo S, Li M, Chen J, Beye A, Soboyejo W . Cell/surface interactions on laser micro-textured titanium-coated silicon surfaces. J Mater Sci Mater Med. 2007; 18(1):9-23. DOI: 10.1007/s10856-006-0658-9. View

18.

McBeath R, Pirone D, Nelson C, Bhadriraju K, Chen C . Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. Dev Cell. 2004; 6(4):483-95. DOI: 10.1016/s1534-5807(04)00075-9. View

19.

Chan E, Yousaf M . A photo-electroactive surface strategy for immobilizing ligands in patterns and gradients for studies of cell polarization. Mol Biosyst. 2008; 4(7):746-53. DOI: 10.1039/b801394b. View

20.

Curtis A, Wilkinson C . Topographical control of cells. Biomaterials. 1998; 18(24):1573-83. DOI: 10.1016/s0142-9612(97)00144-0. View