Interactions of Typical and Atypical Enteropathogenic Escherichia Coli Strains with the Calf Intestinal Mucosa Ex Vivo

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2009 Jul 28

PMID 19633123

Citations 5

Authors

Francis Girard

Francis Dziva

Mark P Stevens

Gad Frankel

Affiliations

Soon will be listed here.

Abstract

Enteropathogenic Escherichia coli (EPEC) can be found in healthy and diarrheic cattle; however, little is known about the role of attaching and effacing (A/E) lesion formation in colonization of bovine intestinal mucosa by such strains. We show that typical and atypical EPEC induce A/E lesions on calf intestinal explants independently of Tir tyrosine phosphorylation and TccP. Our data support the existence of conserved Tir- and TccP-independent mechanisms of A/E lesion formation in a range of hosts and reinforce the zoonotic potential of EPEC in cattle.

Citing Articles

Epithelial maturity influences EPEC-induced desmosomal alterations.

Roxas J, Vedantam G, Viswanathan V Gut Microbes. 2018; 10(2):241-245.

PMID: 30183504 PMC: 6546310. DOI: 10.1080/19490976.2018.1506669.

Citrobacter rodentium mouse model of bacterial infection.

Crepin V, Collins J, Habibzay M, Frankel G Nat Protoc. 2016; 11(10):1851-76.

PMID: 27606775 DOI: 10.1038/nprot.2016.100.

Intimate host attachment: enteropathogenic and enterohaemorrhagic Escherichia coli.

Lai Y, Rosenshine I, Leong J, Frankel G Cell Microbiol. 2013; 15(11):1796-808.

PMID: 23927593 PMC: 4036124. DOI: 10.1111/cmi.12179.

Genome Sequence of Porcine Escherichia coli Strain IMT8073, an Atypical Enteropathogenic E. coli Strain Isolated from a Piglet with Diarrhea.

Semmler T, Eichhorn I, Bethe A, Bauerfeind R, Pickard D, Kingsley R Genome Announc. 2013; 1(4).

PMID: 23908298 PMC: 3731852. DOI: 10.1128/genomeA.00573-13.

Dissecting the role of the Tir:Nck and Tir:IRTKS/IRSp53 signalling pathways in vivo.

Crepin V, Girard F, Schuller S, Phillips A, Mousnier A, Frankel G Mol Microbiol. 2009; 75(2):308-23.

PMID: 19889090 PMC: 2814079. DOI: 10.1111/j.1365-2958.2009.06938.x.

References

Jarvis K, Kaper J . Secretion of extracellular proteins by enterohemorrhagic Escherichia coli via a putative type III secretion system. Infect Immun. 1996; 64(11):4826-9. PMC: 174451. DOI: 10.1128/iai.64.11.4826-4829.1996. View

Aidar-Ugrinovich L, Blanco J, Blanco M, Blanco J, Leomil L, Dahbi G . Serotypes, virulence genes, and intimin types of Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) isolated from calves in São Paulo, Brazil. Int J Food Microbiol. 2007; 115(3):297-306. DOI: 10.1016/j.ijfoodmicro.2006.10.046. View

Giron J, Ho A, Schoolnik G . An inducible bundle-forming pilus of enteropathogenic Escherichia coli. Science. 1991; 254(5032):710-3. DOI: 10.1126/science.1683004. View

Vingadassalom D, Kazlauskas A, Skehan B, Cheng H, Magoun L, Robbins D . Insulin receptor tyrosine kinase substrate links the E. coli O157:H7 actin assembly effectors Tir and EspF(U) during pedestal formation. Proc Natl Acad Sci U S A. 2009; 106(16):6754-9. PMC: 2672544. DOI: 10.1073/pnas.0809131106. View

Frankel G, Phillips A, Rosenshine I, Dougan G, Kaper J, Knutton S . Enteropathogenic and enterohaemorrhagic Escherichia coli: more subversive elements. Mol Microbiol. 1999; 30(5):911-21. DOI: 10.1046/j.1365-2958.1998.01144.x. View

Lacher D, Steinsland H, Blank T, Donnenberg M, Whittam T . Molecular evolution of typical enteropathogenic Escherichia coli: clonal analysis by multilocus sequence typing and virulence gene allelic profiling. J Bacteriol. 2006; 189(2):342-50. PMC: 1797380. DOI: 10.1128/JB.01472-06. View

Hartland E, Batchelor M, Delahay R, Hale C, Matthews S, Dougan G . Binding of intimin from enteropathogenic Escherichia coli to Tir and to host cells. Mol Microbiol. 1999; 32(1):151-8. DOI: 10.1046/j.1365-2958.1999.01338.x. View

Garmendia J, Phillips A, Carlier M, Chong Y, Schuller S, Marches O . TccP is an enterohaemorrhagic Escherichia coli O157:H7 type III effector protein that couples Tir to the actin-cytoskeleton. Cell Microbiol. 2004; 6(12):1167-83. DOI: 10.1111/j.1462-5822.2004.00459.x. View

Ishii S, Meyer K, Sadowsky M . Relationship between phylogenetic groups, genotypic clusters, and virulence gene profiles of Escherichia coli strains from diverse human and animal sources. Appl Environ Microbiol. 2007; 73(18):5703-10. PMC: 2074926. DOI: 10.1128/AEM.00275-07. View

10.

Vlisidou I, Dziva F, La Ragione R, Best A, Garmendia J, Hawes P . Role of intimin-tir interactions and the tir-cytoskeleton coupling protein in the colonization of calves and lambs by Escherichia coli O157:H7. Infect Immun. 2005; 74(1):758-64. PMC: 1346653. DOI: 10.1128/IAI.74.1.758-764.2006. View

11.

Knutton S, Lloyd D, McNeish A . Adhesion of enteropathogenic Escherichia coli to human intestinal enterocytes and cultured human intestinal mucosa. Infect Immun. 1987; 55(1):69-77. PMC: 260281. DOI: 10.1128/iai.55.1.69-77.1987. View

12.

Frankel G, Phillips A, Trabulsi L, Knutton S, Dougan G, Matthews S . Intimin and the host cell--is it bound to end in Tir(s)?. Trends Microbiol. 2001; 9(5):214-8. DOI: 10.1016/s0966-842x(01)02016-9. View

13.

Campellone K, Robbins D, Leong J . EspFU is a translocated EHEC effector that interacts with Tir and N-WASP and promotes Nck-independent actin assembly. Dev Cell. 2004; 7(2):217-28. DOI: 10.1016/j.devcel.2004.07.004. View

14.

Pearson G, Watson C, Hall G, Wray C . Natural infection with an attaching and effacing Escherichia coli in the small and large intestines of a calf with diarrhoea. Vet Rec. 1989; 124(12):297-9. DOI: 10.1136/vr.124.12.297. View

15.

van Diemen P, Dziva F, Stevens M, Wallis T . Identification of enterohemorrhagic Escherichia coli O26:H- genes required for intestinal colonization in calves. Infect Immun. 2005; 73(3):1735-43. PMC: 1064914. DOI: 10.1128/IAI.73.3.1735-1743.2005. View

16.

Frankel G, Phillips A . Attaching effacing Escherichia coli and paradigms of Tir-triggered actin polymerization: getting off the pedestal. Cell Microbiol. 2007; 10(3):549-56. DOI: 10.1111/j.1462-5822.2007.01103.x. View

17.

Weiss S, Ladwein M, Schmidt D, Ehinger J, Lommel S, Stading K . IRSp53 links the enterohemorrhagic E. coli effectors Tir and EspFU for actin pedestal formation. Cell Host Microbe. 2009; 5(3):244-58. DOI: 10.1016/j.chom.2009.02.003. View

18.

Caron E, Crepin V, Simpson N, Knutton S, Garmendia J, Frankel G . Subversion of actin dynamics by EPEC and EHEC. Curr Opin Microbiol. 2006; 9(1):40-5. DOI: 10.1016/j.mib.2005.12.008. View

19.

Kenny B, DeVinney R, Stein M, Reinscheid D, Frey E, Finlay B . Enteropathogenic E. coli (EPEC) transfers its receptor for intimate adherence into mammalian cells. Cell. 1997; 91(4):511-20. DOI: 10.1016/s0092-8674(00)80437-7. View

20.

McDaniel T, Jarvis K, Donnenberg M, Kaper J . A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens. Proc Natl Acad Sci U S A. 1995; 92(5):1664-8. PMC: 42580. DOI: 10.1073/pnas.92.5.1664. View