Evaluation of Three Panels of Monoclonal Antibodies for the Identification of Human Rotavirus VP7 Serotype by ELISA

Overview

Journal Bull World Health Organ

Specialty Public Health

Date 1990 Jan 11

PMID 1963119

Citations 6

Authors

K Y Green

H D JAMES Jr

A Z Kapikian

Affiliations

Soon will be listed here.

Abstract

Three panels of monoclonal antibodies used for rotavirus serotype identification by enzyme-linked immunosorbent assay (ELISA) were evaluated at the National Institutes of Health, USA, to identify antibodies suitable for distribution to laboratories involved in WHO-sponsored trials of rotavirus vaccines. Two of the panels were comparably effective in identifying the serotype of each of the human rotavirus reference strains of serotype 1, 2, or 3. In addition, one of the panels included a monoclonal antibody that was effective in identifying strains of serotype 4. However, two different lots of a third, commercially available panel were not effective in identifying the eight strains representing the four serotypes. A third shipment of this panel was therefore tested using revised instructions and, under these conditions, it was effective in serotyping seven of the eight reference strains. It appears that a battery of monoclonal antibodies for each serotype may be required to identify antigenic variants within a serotype. Additional studies are needed to assess the extent of antigenic variation in rotavirus field strains.

Citing Articles

Rotavirus typing methods and algorithms.

Fischer T, Gentsch J Rev Med Virol. 2004; 14(2):71-82.

PMID: 15027000 PMC: 7169166. DOI: 10.1002/rmv.411.

1998-1999 rotavirus seasons in Juiz de Fora, Minas Gerais, Brazil: detection of an unusual G3P[4] epidemic strain.

Rosa E Silva M, de Carvalho I, Gouvea V J Clin Microbiol. 2002; 40(8):2837-42.

PMID: 12149339 PMC: 120661. DOI: 10.1128/JCM.40.8.2837-2842.2002.

Survey of equine rotaviruses shows conservation of one P genotype in background of two G genotypes.

Isa P, Wood A, Netherwood T, Ciarlet M, Imagawa H, Snodgrass D Arch Virol. 1996; 141(9):1601-12.

PMID: 8893784 DOI: 10.1007/BF01718285.

Serotype analysis of rotaviruses from different locations in Malaysia.

Rasool N, Green K, Kapikian A J Clin Microbiol. 1993; 31(7):1815-9.

PMID: 8394376 PMC: 265638. DOI: 10.1128/jcm.31.7.1815-1819.1993.

Comparisons of rotavirus VP7-typing monoclonal antibodies by competition binding assay.

Raj P, Matson D, Coulson B, Bishop R, Taniguchi K, URASAWA S J Clin Microbiol. 1992; 30(3):704-11.

PMID: 1372622 PMC: 265136. DOI: 10.1128/jcm.30.3.704-711.1992.

References

Wyatt R, James W, BOHL E, Theil K, Saif L, Kalica A . Human rotavirus type 2: cultivation in vitro. Science. 1980; 207(4427):189-91. DOI: 10.1126/science.6243190. View

Taniguchi K, Urasawa T, Morita Y, Greenberg H, URASAWA S . Direct serotyping of human rotavirus in stools by an enzyme-linked immunosorbent assay using serotype 1-, 2-, 3-, and 4-specific monoclonal antibodies to VP7. J Infect Dis. 1987; 155(6):1159-66. DOI: 10.1093/infdis/155.6.1159. View

Albert M, Unicomb L, Barnes G, Bishop R . Cultivation and characterization of rotavirus strains infecting newborn babies in Melbourne, Australia, from 1975 to 1979. J Clin Microbiol. 1987; 25(9):1635-40. PMC: 269297. DOI: 10.1128/jcm.25.9.1635-1640.1987. View

Clark H, Hoshino Y, Bell L, Groff J, Hess G, Bachman P . Rotavirus isolate WI61 representing a presumptive new human serotype. J Clin Microbiol. 1987; 25(9):1757-62. PMC: 269322. DOI: 10.1128/jcm.25.9.1757-1762.1987. View

Green K, Midthun K, Gorziglia M, Hoshino Y, Kapikian A, Chanock R . Comparison of the amino acid sequences of the major neutralization protein of four human rotavirus serotypes. Virology. 1987; 161(1):153-9. DOI: 10.1016/0042-6822(87)90181-4. View

Green K, Sears J, Taniguchi K, Midthun K, Hoshino Y, Gorziglia M . Prediction of human rotavirus serotype by nucleotide sequence analysis of the VP7 protein gene. J Virol. 1988; 62(5):1819-23. PMC: 253238. DOI: 10.1128/JVI.62.5.1819-1823.1988. View

Green K, Hoshino Y, IKEGAMI N . Sequence analysis of the gene encoding the serotype-specific glycoprotein (VP7) of two new human rotavirus serotypes. Virology. 1989; 168(2):429-33. DOI: 10.1016/0042-6822(89)90289-4. View

Nishikawa K, Hoshino Y, Taniguchi K, Green K, Greenberg H, Kapikian A . Rotavirus VP7 neutralization epitopes of serotype 3 strains. Virology. 1989; 171(2):503-15. DOI: 10.1016/0042-6822(89)90620-x. View

Ahmed M, Taniguchi K, Kobayashi N, Urasawa T, WAKASUGI F, Islam M . Characterization by enzyme-linked immunosorbent assay using subgroup- and serotype-specific monoclonal antibodies of human rotavirus obtained from diarrheic patients in Bangladesh. J Clin Microbiol. 1989; 27(7):1678-81. PMC: 267642. DOI: 10.1128/jcm.27.7.1678-1681.1989. View

10.

Beards G, PILFOLD J, Thouless M, Flewett T . Rotavirus serotypes by serum neutralisation. J Med Virol. 1980; 5(3):231-7. DOI: 10.1002/jmv.1890050307. View

11.

Urasawa T, URASAWA S, Taniguchi K . Sequential passages of human rotavirus in MA-104 cells. Microbiol Immunol. 1981; 25(10):1025-35. DOI: 10.1111/j.1348-0421.1981.tb00109.x. View

12.

Wyatt R, Greenberg H, James W, Pittman A, Kalica A, Flores J . Definition of human rotavirus serotypes by plaque reduction assay. Infect Immun. 1982; 37(1):110-5. PMC: 347497. DOI: 10.1128/iai.37.1.110-115.1982. View

13.

Hoshino Y, Wyatt R, Greenberg H, Flores J, Kapikian A . Serotypic similarity and diversity of rotaviruses of mammalian and avian origin as studied by plaque-reduction neutralization. J Infect Dis. 1984; 149(5):694-702. DOI: 10.1093/infdis/149.5.694. View

14.

Hoshino Y, Wyatt R, Flores J, Midthun K, Kapikian A . Serotypic characterization of rotaviruses derived from asymptomatic human neonatal infections. J Clin Microbiol. 1985; 21(3):425-30. PMC: 271678. DOI: 10.1128/jcm.21.3.425-430.1985. View

15.

Matsuno S, Hasegawa A, Mukoyama A, Inouye S . A candidate for a new serotype of human rotavirus. J Virol. 1985; 54(2):623-4. PMC: 254837. DOI: 10.1128/JVI.54.2.623-624.1985. View

16.

Shaw R, Estes M, Greenberg H . Specific enzyme-linked immunoassay for rotavirus serotypes 1 and 3. J Clin Microbiol. 1985; 22(2):286-91. PMC: 268377. DOI: 10.1128/jcm.22.2.286-291.1985. View

17.

Gerna G, Passarani N, Sarasini A, Battaglia M . Characterization of serotypes of human rotavirus strains by solid-phase immune electron microscopy. J Infect Dis. 1985; 152(6):1143-51. DOI: 10.1093/infdis/152.6.1143. View

18.

Coulson B, Tursi J, McAdam W, Bishop R . Derivation of neutralizing monoclonal antibodies to human rotaviruses and evidence that an immunodominant neutralization site is shared between serotypes 1 and 3. Virology. 1986; 154(2):302-12. DOI: 10.1016/0042-6822(86)90456-3. View

19.

Heath R, Birch C, Gust I . Antigenic analysis of rotavirus isolates using monoclonal antibodies specific for human serotypes 1, 2, 3 and 4, and SA11. J Gen Virol. 1986; 67 ( Pt 11):2455-66. DOI: 10.1099/0022-1317-67-11-2455. View

20.

Coulson B, Unicomb L, Pitson G, Bishop R . Simple and specific enzyme immunoassay using monoclonal antibodies for serotyping human rotaviruses. J Clin Microbiol. 1987; 25(3):509-15. PMC: 265970. DOI: 10.1128/jcm.25.3.509-515.1987. View