Molecular Characterization and Therapeutic Potential of a Marine Bacterium Pseudoalteromonas Sp. KMM 701 Alpha-galactosidase

Overview

Journal Mar Biotechnol (NY)

Specialties Biology
Biotechnology

Date 2009 Jul 25

PMID 19629597

Citations 8

Authors

Larissa A Balabanova

Irina Yu Bakunina

Olga I Nedashkovskaya

Ilona D Makarenkova

Tatiana S Zaporozhets

Natalia N Besednova

Tatiana N Zvyagintseva

Valery A Rasskazov

Affiliations

Soon will be listed here.

Abstract

An alpha-galactosidase capable of converting B red blood cells into the universal blood type cells at the neutral pH was produced by a novel obligate marine bacterium strain KMM 701 (VKM B-2135 D). The organism is heterotrophic, aerobic, and halophilic and requires Na+ ions and temperature up to 34 degrees C for its growth. The strain has a unique combination of polysaccharide-degrading enzymes. Its single intracellular alpha-galactosidase exceeded other glycoside hydrolases in the level of expression up to 20-fold. The alpha-galactosidase was purified to determine the N-terminal amino acid sequences and new activities. It was found to inhibit Corynebacterium diphtheria adhesion to host buccal epithelium cell surfaces with high effectiveness. The nucleotide sequence of the homodimeric alpha-galactosidase indicates that its subunit is composed of 710 amino acid residues with a calculated Mr of 80,055. This alpha-galactosidase shares structural property with 36 family glycoside hydrolases. The properties of the enzyme are likely to be highly beneficial for medicinal purposes.

Citing Articles

Cold-Active Enzymes and Their Potential Industrial Applications-A Review.

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Genome-Based Classification of Strain 16-SW-7, a Marine Bacterium Capable of Converting B Red Blood Cells, as and Proposal to Reclassify as a Later Heterotypic Synonym of .

Nedashkovkaya O, Kim S, Balabanova L, Zhukova N, Son O, Tekutyeva L Front Microbiol. 2022; 12:809431.

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Effects of Sponge-Derived Alkaloids on Activities of the Bacterial α-D-Galactosidase and Human Cancer Cell α-N-Acetylgalactosaminidase.

Utkina N, Likhatskaya G, Malyarenko O, Ermakova S, Balabanova L, Slepchenko L Biomedicines. 2021; 9(5).

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Effect of Pentacyclic Guanidine Alkaloids from the Sponge on Activity of α-Glycosidases from Marine Bacteria.

Bakunina I, Likhatskaya G, Slepchenko L, Balabanova L, Tekutyeva L, Son O Mar Drugs. 2019; 17(1).

PMID: 30609674 PMC: 6356649. DOI: 10.3390/md17010022.

Characterization of Properties and Transglycosylation Abilities of Recombinant α-Galactosidase from Cold-Adapted Marine Bacterium KMM 701 and Its C494N and D451A Mutants.

Bakunina I, Slepchenko L, Anastyuk S, Isakov V, Likhatskaya G, Kim N Mar Drugs. 2018; 16(10).

PMID: 30250010 PMC: 6213131. DOI: 10.3390/md16100349.

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