The Analysis of Tryptase in Serum of Sarcoidosis Patients

Overview

Journal Inflammation

Specialties Allergy & Immunology
Pathology

Date 2009 Jul 8

PMID 19582562

Citations 6

Authors

Elena Bargagli

Angela Mazzi

Fabrizio Mezzasalma

Anna Perrone

Carmela Olivieri

Antje Prasse

Nicola Bianchi

Maria G Pieroni

Paola Rottoli

Affiliations

Soon will be listed here.

Abstract

Sarcoidosis is a systemic granulomatous disease of unknown etiology characterized by activation of macrophages and T lymphocytes. Relatively little is known about the role of mast cells and their mediators in the pathogenesis of sarcoidosis. Tryptase is an enzyme produced by activated mast cells, regarded as a marker of mast cell activation. To analyse tryptase concentrations in serum of sarcoidosis patients in an attempt to define the role of tryptase and mast cells in the pathogenesis of sarcoidosis and to evaluate the potential of tryptase as marker of disease severity. Quantitative analysis of tryptase concentrations was performed in serum of patients with stable sarcoidosis (n = 12), progressive sarcoidosis (n = 23) and controls (n = 13). Patients enrolled in the study had been monitored at Siena Regional Referral Centre for Sarcoidosis from onset for at least 12 months. Significantly higher concentrations of tryptase were found in peripheral blood of sarcoidosis patients (6.08 +/- 3.98 microg/l) than controls (2.96 +/- 1.75microg/l; p = 0.012). Patients with progressive disease showed the highest tryptase concentrations in serum. Tryptase and mast cells may be involved in the immunopathogenesis of sarcoidosis and further studies are required to understand if tryptase may represent a marker of sarcoidosis severity.

Citing Articles

Clinical phenotyping in sarcoidosis management.

Rana G, DAlessandro M, Rizzi L, Bergantini L, Cameli P, Vozza A Sarcoidosis Vasc Diffuse Lung Dis. 2021; 38(2):e2021007.

PMID: 34316252 PMC: 8288209. DOI: 10.36141/svdld.v38i2.10423.

Catch the rainbow: Prognostic factor of sarcoidosis.

Kobak S Lung India. 2020; 37(5):425-432.

PMID: 32883904 PMC: 7857382. DOI: 10.4103/lungindia.lungindia_380_19.

Cathepsin S, a new serum biomarker of sarcoidosis discovered by transcriptome analysis of alveolar macrophages.

Tanaka H, Yamaguchi E, Asai N, Yokoi T, Nishimura M, Nakao H Sarcoidosis Vasc Diffuse Lung Dis. 2020; 36(2):141-147.

PMID: 32476947 PMC: 7247107. DOI: 10.36141/svdld.v36i2.7620.

PDE3 Inhibition Reduces Epithelial Mast Cell Numbers in Allergic Airway Inflammation and Attenuates Degranulation of Basophils and Mast Cells.

Beute J, Ganesh K, Nastiti H, Hoogenboom R, Bos V, Folkerts J Front Pharmacol. 2020; 11:470.

PMID: 32425769 PMC: 7206980. DOI: 10.3389/fphar.2020.00470.

Use of discriminant analysis in assessing pulmonary function worsening in patients with sarcoidosis by a panel of inflammatory biomarkers.

Paone G, Leone A, Batzella S, Conti V, Belli F, De Marchis L Inflamm Res. 2012; 62(3):325-32.

PMID: 23262919 DOI: 10.1007/s00011-012-0585-9.

References

Bargagli E, Bigliazzi C, Leonini A, Nikiforakis N, Perari M, Rottoli P . Tryptase concentrations in bronchoalveolar lavage from patients with chronic eosinophilic pneumonia. Clin Sci (Lond). 2004; 108(3):273-6. DOI: 10.1042/CS20040178. View

Caughey G . Tryptase genetics and anaphylaxis. J Allergy Clin Immunol. 2006; 117(6):1411-4. PMC: 2271076. DOI: 10.1016/j.jaci.2006.02.026. View

Edwards S, Cruz A, Donnelly S, Dazin P, Schulman E, Jones K . c-Kit immunophenotyping and metalloproteinase expression profiles of mast cells in interstitial lung diseases. J Pathol. 2005; 206(3):279-90. DOI: 10.1002/path.1780. View

Kantrow S, Meyer K, Kidd P, Raghu G . The CD4/CD8 ratio in BAL fluid is highly variable in sarcoidosis. Eur Respir J. 1998; 10(12):2716-21. DOI: 10.1183/09031936.97.10122716. View

Drent M, Jacobs J, de Vries J, Lamers R, Liem I, Wouters E . Does the cellular bronchoalveolar lavage fluid profile reflect the severity of sarcoidosis?. Eur Respir J. 1999; 13(6):1338-44. DOI: 10.1183/09031936.99.13613459. View

HEARD B, Dewar A, Corrin B . Apposition of fibroblasts to mast cells and lymphocytes in normal human lung and in cryptogenic fibrosing alveolitis. Ultrastructure and cell perimeter measurements. J Pathol. 1992; 166(3):303-10. DOI: 10.1002/path.1711660314. View

Rottoli P, Rottoli L, Perari M, Collodoro A, Carriero G, Bianco S . Mast cells in bronchoalveolar lavage in sarcoidosis: correlation with alveolar lymphocytes. Respiration. 1988; 54 Suppl 1:42-8. DOI: 10.1159/000195476. View

Miller H, Pemberton A . Tissue-specific expression of mast cell granule serine proteinases and their role in inflammation in the lung and gut. Immunology. 2002; 105(4):375-90. PMC: 1782685. DOI: 10.1046/j.1365-2567.2002.01375.x. View

Ziegenhagen M, Rothe M, Schlaak M, Muller-Quernheim J . Bronchoalveolar and serological parameters reflecting the severity of sarcoidosis. Eur Respir J. 2003; 21(3):407-13. DOI: 10.1183/09031936.03.00010403. View

10.

Schwartz L, Metcalfe D, Miller J, Earl H, Sullivan T . Tryptase levels as an indicator of mast-cell activation in systemic anaphylaxis and mastocytosis. N Engl J Med. 1987; 316(26):1622-6. DOI: 10.1056/NEJM198706253162603. View

11.

Bargagli E, Mazzi A, Rottoli P . Markers of inflammation in sarcoidosis: blood, urine, BAL, sputum, and exhaled gas. Clin Chest Med. 2008; 29(3):445-58, viii. DOI: 10.1016/j.ccm.2008.03.004. View

12.

Amano H, Kurosawa M, Ishikawa O, Chihara J, Miyachi Y . Mast cells in the cutaneous lesions of sarcoidosis: their subtypes and the relationship to systemic manifestations. J Dermatol Sci. 2000; 24(1):60-6. DOI: 10.1016/s0923-1811(00)00085-2. View

13.

Rasp G, HOCHSTRASSER K . Tryptase in nasal fluid is a useful marker of allergic rhinitis. Allergy. 1993; 48(2):72-4. DOI: 10.1111/j.1398-9995.1993.tb00688.x. View

14.

Eklund A, van Hage-Hamsten M, Skold C, JOHANSSON S . Elevated levels of tryptase in bronchoalveolar lavage fluid from patients with sarcoidosis. Sarcoidosis. 1993; 10(1):12-7. View

15.

Huang C, De Sanctis G, OBrien P, Mizgerd J, Friend D, Drazen J . Evaluation of the substrate specificity of human mast cell tryptase beta I and demonstration of its importance in bacterial infections of the lung. J Biol Chem. 2001; 276(28):26276-84. DOI: 10.1074/jbc.M102356200. View

16.

Baughman R, Lower E, du Bois R . Sarcoidosis. Lancet. 2003; 361(9363):1111-8. DOI: 10.1016/S0140-6736(03)12888-7. View

17.

Hunninghake G, Gilbert S, Pueringer R, Dayton C, Floerchinger C, Helmers R . Outcome of the treatment for sarcoidosis. Am J Respir Crit Care Med. 1994; 149(4 Pt 1):893-8. DOI: 10.1164/ajrccm.149.4.8143052. View

18.

Hunninghake G, Crystal R . Pulmonary sarcoidosis: a disorder mediated by excess helper T-lymphocyte activity at sites of disease activity. N Engl J Med. 1981; 305(8):429-34. DOI: 10.1056/NEJM198108203050804. View

19.

Kawatani K, Kondo M, Tamaoki J, Tagaya E, Nagai A . [The clinical significance of mast cell tryptase in bronchial alveolar lavage fluid in interstitial lung diseases]. Nihon Kokyuki Gakkai Zasshi. 2007; 45(11):848-55. View

20.

Scadding J . Prognosis of intrathoracic sarcoidosis in England. A review of 136 cases after five years' observation. Br Med J. 1961; 2(5261):1165-72. PMC: 1970202. DOI: 10.1136/bmj.2.5261.1165. View