Time Dependent Gene Expression Changes in the Liver of Mice Treated with Benzene

Overview

Journal Biomark Insights

Publisher Sage Publications

Date 2009 Jul 7

PMID 19578504

Citations 8

Authors

Han-Jin Park

Jung Hwa Oh

Seokjoo Yoon

S V S Rana

Affiliations

Soon will be listed here.

Abstract

Benzene is used as a general purpose solvent. Benzene metabolism starts from phenol and ends with p-benzoquinone and o-benzoquinone. Liver injury inducted by benzene still remains a toxicologic problem. Tumor related genes and immune responsive genes have been studied in patients suffering from benzene exposure. However, gene expression profiles and pathways related to its hepatotoxicity are not known. This study reports the results obtained in the liver of BALB/C mice (SLC, Inc., Japan) administered 0.05 ml/100 g body weight of 2% benzene for six days. Serum, ALT, AST and ALP were determined using automated analyzer (Fuji., Japan). Histopathological observations were made to support gene expression data. c-DNA microarray analyses were performed using Affymetrix Gene-chip system. After six days of benzene exposure, twenty five genes were down regulated whereas nineteen genes were up-regulated. These gene expression changes were found to be related to pathways of biotransformation, detoxification, apoptosis, oxidative stress and cell cycle. It has been shown for the first time that genes corresponding to circadian rhythms are affected by benzene. Results suggest that gene expression profile might serve as potential biomarkers of hepatotoxicity during benzene exposure.

Citing Articles

Associations between specific volatile organic chemical exposures and cardiovascular disease risks: insights from NHANES.

Han S, Xie M, Cheng S, Han Y, Li P, Guo J Front Public Health. 2024; 12:1378444.

PMID: 38846604 PMC: 11153666. DOI: 10.3389/fpubh.2024.1378444.

Assessing volatile organic compounds exposure and prostate-specific antigen: National Health and Nutrition Examination Survey, 2001-2010.

Wei C, Chen Y, Yang Y, Ni D, Huang Y, Wang M Front Public Health. 2022; 10:957069.

PMID: 35968491 PMC: 9372286. DOI: 10.3389/fpubh.2022.957069.

Volatile organic compounds: A proinflammatory activator in autoimmune diseases.

Ogbodo J, Arazu A, Iguh T, Onwodi N, Ezike T Front Immunol. 2022; 13:928379.

PMID: 35967306 PMC: 9373925. DOI: 10.3389/fimmu.2022.928379.

Blood BTEXS and heavy metal levels are associated with liver injury and systemic inflammation in Gulf states residents.

Werder E, Beier J, Sandler D, Falkner K, Gripshover T, Wahlang B Food Chem Toxicol. 2020; 139:111242.

PMID: 32205228 PMC: 7368391. DOI: 10.1016/j.fct.2020.111242.

Interaction of volatile organic compounds and underlying liver disease: a new paradigm for risk.

Lang A, Beier J Biol Chem. 2018; 399(11):1237-1248.

PMID: 29924722 PMC: 6181143. DOI: 10.1515/hsz-2017-0324.

References

Kim E, Kang B, Kim T . Inhibition of interleukin-12 production in mouse macrophages by hydroquinone, a reactive metabolite of benzene, via suppression of nuclear factor-kappaB binding activity. Immunol Lett. 2005; 99(1):24-9. DOI: 10.1016/j.imlet.2004.11.025. View

Heijne W, Jonker D, Stierum R, van Ommen B, Groten J . Toxicogenomic analysis of gene expression changes in rat liver after a 28-day oral benzene exposure. Mutat Res. 2005; 575(1-2):85-101. DOI: 10.1016/j.mrfmmm.2005.02.003. View

Henderson R . Species differences in the metabolism of benzene. Environ Health Perspect. 1996; 104 Suppl 6:1173-5. PMC: 1469720. DOI: 10.1289/ehp.961041173. View

Ross D . The role of metabolism and specific metabolites in benzene-induced toxicity: evidence and issues. J Toxicol Environ Health A. 2000; 61(5-6):357-72. DOI: 10.1080/00984100050166361. View

Snyder R . Benzene and leukemia. Crit Rev Toxicol. 2002; 32(3):155-210. DOI: 10.1080/20024091064219. View

Wang H, Bi Y, Wang C, Tao N, Xia Y, Zhang L . [cDNA microarray and cluster analysis to identify the significance of immune genes associated with benzene poisoning]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2005; 23(4):260-2. View

Chen L, Bi Y, Tao N, Wang H, Xia Y, Ma Q . [cDNA microarray to identify the significance of DNA replication and damage repair genes associated with benzene poisoning]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2005; 23(4):248-51. View

Chen H, Eastmond D . Synergistic increase in chromosomal breakage within the euchromatin induced by an interaction of the benzene metabolites phenol and hydroquinone in mice. Carcinogenesis. 1995; 16(8):1963-9. DOI: 10.1093/carcin/16.8.1963. View

Yoon B, Li G, Kitada K, Kawasaki Y, Igarashi K, Kodama Y . Mechanisms of benzene-induced hematotoxicity and leukemogenicity: cDNA microarray analyses using mouse bone marrow tissue. Environ Health Perspect. 2003; 111(11):1411-20. PMC: 1241634. DOI: 10.1289/ehp.6164. View

10.

Valentine J, Lee S, Seaton M, Asgharian B, Farris G, Corton J . Reduction of benzene metabolism and toxicity in mice that lack CYP2E1 expression. Toxicol Appl Pharmacol. 1996; 141(1):205-13. DOI: 10.1006/taap.1996.0277. View

11.

Waters M, Olden K, Tennant R . Toxicogenomic approach for assessing toxicant-related disease. Mutat Res. 2003; 544(2-3):415-24. DOI: 10.1016/j.mrrev.2003.06.014. View

12.

Lee E, Garner C . Effects of benzene on DNA strand breaks in vivo versus benzene metabolite-induced DNA strand breaks in vitro in mouse bone marrow cells. Toxicol Appl Pharmacol. 1991; 108(3):497-508. DOI: 10.1016/0041-008x(91)90096-w. View

13.

Leung P, Harrison R . Evaluation of personal exposure to monoaromatic hydrocarbons. Occup Environ Med. 1998; 55(4):249-57. PMC: 1757578. DOI: 10.1136/oem.55.4.249. View

14.

Farris G, Robinson S, Gaido K, Wong B, Wong V, Hahn W . Benzene-induced hematotoxicity and bone marrow compensation in B6C3F1 mice. Fundam Appl Toxicol. 1997; 36(2):119-29. DOI: 10.1006/faat.1997.2293. View

15.

Wang H, Bi Y, Tao N, Wang C . [Differentially expressed genes of cell signal transduction associated with benzene poisoning by cDNA microarray]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2005; 23(4):252-5. View

16.

Smith M, Vermeulen R, Li G, Zhang L, Lan Q, Hubbard A . Use of 'Omic' technologies to study humans exposed to benzene. Chem Biol Interact. 2005; 153-154:123-7. DOI: 10.1016/j.cbi.2005.03.017. View

17.

Gut I, Nedelcheva V, Soucek P, Stopka P, Vodicka P, Gelboin H . The role of CYP2E1 and 2B1 in metabolic activation of benzene derivatives. Arch Toxicol. 1996; 71(1-2):45-56. DOI: 10.1007/s002040050357. View

18.

Niculescu R, Bradford H, Colman R, Kalf G . Inhibition of the conversion of pre-interleukins-1 alpha and 1 beta to mature cytokines by p-benzoquinone, a metabolite of benzene. Chem Biol Interact. 1995; 98(3):211-22. DOI: 10.1016/0009-2797(95)03647-4. View

19.

Starek A, Lepiarz W, Baran J . [Susceptibility of rats to benzene during morning and evening]. Folia Med Cracov. 1990; 31(4):279-87. View

20.

Minami K, Saito T, Narahara M, Tomita H, Kato H, Sugiyama H . Relationship between hepatic gene expression profiles and hepatotoxicity in five typical hepatotoxicant-administered rats. Toxicol Sci. 2005; 87(1):296-305. DOI: 10.1093/toxsci/kfi235. View