Intragastric Administration of Attenuated Salmonella Typhimurium Harbouring Transmissible Gastroenteritis Virus (TGEV) DNA Vaccine Induced Specific Antibody Production

Overview

Journal Vaccine

Specialty Allergy & Immunology

Date 2009 Jul 4

PMID 19573642

Citations 7

Authors

Heng Yang

Sanjie Cao

Xiaobo Huang

Jiawen Liu

Ying Tang

Xintian Wen

Affiliations

Soon will be listed here.

Abstract

Attenuated Salmonella typhimurium was selected as a transgenic vehicle for the development of live mucosal vaccines against transmissible gastroenteritis virus (TGEV). A 2.2kb DNA fragment, encoding for N-terminal domain glycoprotein S of TGEV, was amplified by RT-PCR and cloned into eukaryotic expression vector pVAX1. The recombinant plasmid pVAX-S was transformed by electroporation into attenuated S. typhimurium SL7207, the expression and translation of the pVAX-S delivered by recombinant S. typhimurium SL7207 (pVAX-S) was detected in vitro and in vivo respectively. BALB/c mice were inoculated orally with SL7207 (pVAX-S) at different dosages, the bacterium was safe to mice at dosage of 2x10(9)CFU and eventually eliminated from the spleen and liver at week 4 post-immunization. Mice immunized with different dosages of SL7207 (pVAX-S) elicited specific anti-TGEV local mucosal and humoral responses as measured by indirect ELISA assay. Moreover, the immunogenicity of the DNA vaccine was highly dependent on the dosage of the attenuated bacteria used for oral administration, 10(9)CFU dosage group showed higher antibody response than 10(8)CFU and 10(7)CFU dosages groups during week 4-8 post-immunization. The results indicated that attenuated S. typhimurium could be used as a delivery vector for oral immunization of TGEV DNA vaccine.

Citing Articles

Recombinant Live-Attenuated Vaccine for Veterinary Use.

Chagas D, Santos F, de Oliveira N, Bohn T, Dellagostin O Vaccines (Basel). 2025; 12(12.

PMID: 39771981 PMC: 11680399. DOI: 10.3390/vaccines12121319.

Oral Vaccination with a DNA Vaccine Encoding Capsid Protein of Duck Tembusu Virus Induces Protection Immunity.

Huang J, Shen H, Jia R, Wang M, Chen S, Zhu D Viruses. 2018; 10(4).

PMID: 29642401 PMC: 5923474. DOI: 10.3390/v10040180.

Construction of a bivalent DNA vaccine co-expressing S genes of transmissible gastroenteritis virus and porcine epidemic diarrhea virus delivered by attenuated Salmonella typhimurium.

Zhang Y, Zhang X, Liao X, Huang X, Cao S, Wen X Virus Genes. 2016; 52(3):354-64.

PMID: 26980672 DOI: 10.1007/s11262-016-1316-z.

Immunogenicity of transmissible gastroenteritis virus (TGEV) M gene delivered by attenuated Salmonella typhimurium in mice.

Qing Y, Liu J, Huang X, Li Y, Zhang Y, Chen J Virus Genes. 2016; 52(2):218-27.

PMID: 26837896 PMC: 7088643. DOI: 10.1007/s11262-016-1296-z.

Live bacterial vaccine vectors: an overview.

da Silva A, Zangirolami T, Novo-Mansur M, de Campos Giordano R, Martins E Braz J Microbiol. 2015; 45(4):1117-29.

PMID: 25763014 PMC: 4323283. DOI: 10.1590/s1517-83822014000400001.

References

Saif L . Mucosal immunity: an overview and studies of enteric and respiratory coronavirus infections in a swine model of enteric disease. Vet Immunol Immunopathol. 1996; 54(1-4):163-9. PMC: 7119851. DOI: 10.1016/s0165-2427(96)05702-9. View

Tuboly T, Yu W, Bailey A, Degrandis S, Du S, Erickson L . Immunogenicity of porcine transmissible gastroenteritis virus spike protein expressed in plants. Vaccine. 2000; 18(19):2023-8. DOI: 10.1016/s0264-410x(99)00525-3. View

Delmas B, Gelfi J, LHaridon R, Vogel L, Sjostrom H, Noren O . Aminopeptidase N is a major receptor for the entero-pathogenic coronavirus TGEV. Nature. 1992; 357(6377):417-20. PMC: 7095137. DOI: 10.1038/357417a0. View

Flo J, Tisminetzky S, Baralle F . Oral transgene vaccination mediated by attenuated Salmonellae is an effective method to prevent Herpes simplex virus-2 induced disease in mice. Vaccine. 2001; 19(13-14):1772-82. DOI: 10.1016/s0264-410x(00)00375-3. View

Li L, Fang W, Li J, Huang Y, Yu L . Oral DNA vaccination with the polyprotein gene of infectious bursal disease virus (IBDV) delivered by the attenuated Salmonella elicits protective immune responses in chickens. Vaccine. 2006; 24(33-34):5919-27. DOI: 10.1016/j.vaccine.2006.04.057. View

Tuboly T, Nagy E, Dennis J, Derbyshire J . Immunogenicity of the S protein of transmissible gastroenteritis virus expressed in baculovirus. Arch Virol. 1994; 137(1-2):55-67. PMC: 7087137. DOI: 10.1007/BF01311173. View

Anton I, Gonzalez S, Bullido M, Corsin M, Risco C, Langeveld J . Cooperation between transmissible gastroenteritis coronavirus (TGEV) structural proteins in the in vitro induction of virus-specific antibodies. Virus Res. 1996; 46(1-2):111-24. PMC: 7134021. DOI: 10.1016/s0168-1702(96)01390-1. View

Al-Ramadi B, Adeghate E, Mustafa N, Ponery A, Fernandez-Cabezudo M . Cytokine expression by attenuated intracellular bacteria regulates the immune response to infection: the Salmonella model. Mol Immunol. 2002; 38(12-13):931-40. DOI: 10.1016/s0161-5890(02)00020-2. View

Ho P, Kwang J, Lee Y . Intragastric administration of Lactobacillus casei expressing transmissible gastroentritis coronavirus spike glycoprotein induced specific antibody production. Vaccine. 2005; 23(11):1335-42. PMC: 7115493. DOI: 10.1016/j.vaccine.2004.09.015. View

10.

Medina E, Guzman C . Use of live bacterial vaccine vectors for antigen delivery: potential and limitations. Vaccine. 2001; 19(13-14):1573-80. DOI: 10.1016/s0264-410x(00)00354-6. View

11.

Saif L, van Cott J, Brim T . Immunity to transmissible gastroenteritis virus and porcine respiratory coronavirus infections in swine. Vet Immunol Immunopathol. 1994; 43(1-3):89-97. PMC: 7119820. DOI: 10.1016/0165-2427(94)90124-4. View

12.

Laude H, Rasschaert D, Delmas B, Godet M, Gelfi J, Charley B . Molecular biology of transmissible gastroenteritis virus. Vet Microbiol. 1990; 23(1-4):147-54. PMC: 7117338. DOI: 10.1016/0378-1135(90)90144-k. View

13.

Jimenez G, Correa I, Melgosa M, Bullido M, Enjuanes L . Critical epitopes in transmissible gastroenteritis virus neutralization. J Virol. 1986; 60(1):131-9. PMC: 253910. DOI: 10.1128/JVI.60.1.131-139.1986. View

14.

Lamphear B, Jilka J, Kesl L, Welter M, Howard J, Streatfield S . A corn-based delivery system for animal vaccines: an oral transmissible gastroenteritis virus vaccine boosts lactogenic immunity in swine. Vaccine. 2004; 22(19):2420-4. PMC: 7126512. DOI: 10.1016/j.vaccine.2003.11.066. View

15.

Pan Z, Jiao X, Huang J, Yin Y, Tang L, Zhang H . [Safety and efficacy of attenuated Salmonella typhimurium harbouring DNA vaccine against Newcastle disease virus]. Wei Sheng Wu Xue Bao. 2006; 45(6):937-41. View

16.

Lamphear B, Streatfield S, Jilka J, Brooks C, Barker D, Turner D . Delivery of subunit vaccines in maize seed. J Control Release. 2002; 85(1-3):169-80. PMC: 7127645. DOI: 10.1016/s0168-3659(02)00282-1. View

17.

Weiss S . Transfer of eukaryotic expression plasmids to mammalian hosts by attenuated Salmonella spp. Int J Med Microbiol. 2003; 293(1):95-106. DOI: 10.1078/1438-4221-00248. View

18.

Smerdou C, Urniza A, Curtis 3rd R, Enjuanes L . Characterization of transmissible gastroenteritis coronavirus S protein expression products in avirulent S. typhimurium delta cya delta crp: persistence, stability and immune response in swine. Vet Microbiol. 1996; 48(1-2):87-100. PMC: 7117405. DOI: 10.1016/0378-1135(95)00141-7. View

19.

Hu S, BRUSZEWSKI J, Smalling R, Browne J . Studies of TGEV spike protein gp195 expressed in E. coli and by a TGE-vaccinia virus recombinant. Adv Exp Med Biol. 1985; 185:63-82. DOI: 10.1007/978-1-4684-7974-4_4. View

20.

Qu D, Wang S, Cai W, Du A . Protective effect of a DNA vaccine delivered in attenuated Salmonella typhimurium against Toxoplasma gondii infection in mice. Vaccine. 2008; 26(35):4541-8. DOI: 10.1016/j.vaccine.2008.06.030. View