Evaluation of Target Specificity of Antibacterial Agents Using Staphylococcus Aureus DdlA Mutants and D-cycloserine in a Silkworm Infection Model

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 2009 Jun 24

PMID 19546371

Citations 3

Authors

Kenji Kurokawa

Hiroshi Hamamoto

Miki Matsuo

Satoshi Nishida

Noriko Yamane

Bok Luel Lee

Kazuhisa Murakami

Hideki Maki

Kazuhisa Sekimizu

Affiliations

Soon will be listed here.

Abstract

The availability of a silkworm larva infection model to evaluate the therapeutic effectiveness of antibiotics was examined. The 50% effective doses (ED50) of D-cycloserine against the Staphylococcus aureus ddlA mutant-mediated killing of larvae were remarkably lower than those against the parental strain-mediated killing of larvae. Changes in MICs and ED50 of other antibiotics were negligible, suggesting that these alterations are d-cycloserine selective. Therefore, this model is useful for selecting desired compounds based on their therapeutic effectiveness during antibiotic development.

Citing Articles

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Advantages of the Silkworm As an Animal Model for Developing Novel Antimicrobial Agents.

Panthee S, Paudel A, Hamamoto H, Sekimizu K Front Microbiol. 2017; 8:373.

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