Nucleostemin As a Possible Progenitor Marker of Corneal Epithelial Cells

Overview

Journal Mol Vis

Specialties Cell Biology
Molecular Biology
Ophthalmology

Date 2009 Jun 13

PMID 19521549

Citations 5

Authors

Motoko Kawashima

Tetsuya Kawakita

Satoru Yoshida

Shigeto Shimmura

Kazuo Tsubota

Affiliations

Soon will be listed here.

Abstract

Purpose: Nucleostemin, a nuclear protein involved in the regulation of cell cycle and proliferation, is a candidate marker for various stem cells. We examined the expression of nucleostemin as a marker of differentiation and senescence in corneal epithelial progenitor cells.

Methods: Nucleostemin expression in normal mouse corneal epithelium was examined by RT-PCR, as well as in cultured mouse corneal epithelial cells by immunohistochemistry. Co-expression with the progenitor markers p63 and Bmi-1, cytokeratin 14 and 19, the proliferating marker Ki67, differentiation markers cytokeratin 12 and involucrin, and with the senescent marker SA-beta-gal were examined by immunohistochemistry. Correlation of cellular size and nucleostemin expression was also examined.

Results: Nucleostemin expression was detected in mouse corneal epithelium by RT-PCR. Immunohistochemistry revealed that nucleostemin was expressed predominantly in basal and suprabasal cells of the whole cornea. The expression of nucleostemin was not associated with the expression of Ki67, K14, and K19, but with the expression of Bmi-1 and particularly with p63. Nucleostemin was not co-expressed with SA-beta-gal in same cell.

Conclusions: Nucleostemin can be used as a progenitor marker analogous to p63.

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Immunohistochemical study of doublecortin and nucleostemin in canine brain.

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Comparison of telomere length and association with progenitor cell markers in lacrimal gland between Sjögren syndrome and non-Sjögren syndrome dry eye patients.

Kawashima M, Kawakita T, Maida Y, Kamoi M, Ogawa Y, Shimmura S Mol Vis. 2011; 17:1397-404.

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