Role of Nucleostemin in Growth Regulation of Gastric Cancer, Liver Cancer and Other Malignancies

Overview

Journal World J Gastroenterol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2004 Apr 28

PMID 15112336

Citations 35

Authors

Si-Jin Liu

Zi-Wei Cai

Ya-Jun Liu

Mei-Yu Dong

Li-Qiu Sun

Guo-Fa Hu

Ying-Yun Wei

Wei-De Lao

Affiliations

Soon will be listed here.

Abstract

Aim: To examine the role of nucleostemin in the growth regulation of gastric cancer, liver cancer and other cancers.

Methods: RT-PCR was used to clone the fragment of nucleostemin cDNA from HEK 293 cells. Eighteen kinds of malignant tumor tissues including gastric adenocarcinoma and liver cancer tissues, 3 kinds of benign tumor tissues, 3 kinds of benign hyperplastic tissues and normal tissues were employed to examine nucleostemin gene expression by RT-PCR, Slot blot, Northern blot and in situ hybridization.

Results: We successfully cloned a 570 bp fragment of nucleostemin-cDNA from HEK-293 cells. All detected malignant tumor tissues, benign tumor tissues, and benign hyperplastic tissues had high levels of nucleostemin expression. Nucleostemin was also expressed in human placenta tissue at a high level. In terminally differentiated normal human adult kidney and mammary gland tissues, no nucleostemin expression could be detected.

Conclusion: Nucleostemin can help regulate the proliferation of both cancer cells and stem cells. It might play an important role in the growth regulation of gastric cancer, liver cancer and other cancers.

Citing Articles

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Ribosomal L1 domain-containing protein 1 coordinates with HDM2 to negatively regulate p53 in human colorectal Cancer cells.

Ding L, Zhang Z, Zhao C, Chen L, Chen Z, Zhang J J Exp Clin Cancer Res. 2021; 40(1):245.

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Guanine nucleotide binding protein like-1 (GNL1) promotes cancer cell proliferation and survival through AKT/p21 signaling cascade.

Krishnan R, Murugiah M, Lakshmi N, Mahalingam S Mol Biol Cell. 2020; 31(26):2904-2919.

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Interplay between human nucleolar GNL1 and RPS20 is critical to modulate cell proliferation.

Krishnan R, Boddapati N, Mahalingam S Sci Rep. 2018; 8(1):11421.

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Upregulation of GNL3 expression promotes colon cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition via the Wnt/β-catenin signaling pathway.

Tang X, Zha L, Li H, Liao G, Huang Z, Peng X Oncol Rep. 2017; 38(4):2023-2032.

PMID: 28849076 PMC: 5652940. DOI: 10.3892/or.2017.5923.

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