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Rapid Biochemical Tests for Characterization of the Mycoplasmatales

Overview
Specialty Microbiology
Date 1977 May 1
PMID 194917
Citations 8
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Abstract

Methods are described for the rapid detection of beta-D-glucosidase and phosphatase in mycoplasma cultures using fluorogenic 4-methylumbelliferone substrates. These methods were applied to a selection of mycoplasma cultures and were compared with the conventionally used tests for these enzymes. Results were similar by both methods, but the fluorogenic tests could be read after 1 h, whereas the conventional tests took several days.

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References
1.
Erno H, Stipkovits L . Bovine mycoplasmas: cultural and biochemical studies. II. Acta Vet Scand. 1973; 14(3):450-63. PMC: 8559856. View

2.
FABRICANT J, Freundt E . Importance of extension and standardization of laboratory tests for the identification and classification of mycoplasma. Ann N Y Acad Sci. 1967; 143(1):50-8. DOI: 10.1111/j.1749-6632.1967.tb27643.x. View

3.
Jacks T, Kircher H . Fluorometric assay for the hydrolytic activity of lipase using fatty acyl esters of 4-methylumbelliferone. Anal Biochem. 1967; 21(2):279-85. DOI: 10.1016/0003-2697(67)90190-x. View

4.
Bradbury J, Oriel C, Jordan F . Simple method for immunofluorescent identification of mycoplasma colonies. J Clin Microbiol. 1976; 3(4):449-52. PMC: 274322. DOI: 10.1128/jcm.3.4.449-452.1976. View

5.
Maddocks J, Greenan M . A rapid method for identifying bacterial enzymes. J Clin Pathol. 1975; 28(8):686-7. PMC: 475802. DOI: 10.1136/jcp.28.8.686. View