Utility of a Protein Fraction with Cathepsin L-Like Activity Purified from Cysticercus Fluid of Taenia Solium in the Diagnosis of Human Cysticercosis

Overview

Journal Am J Trop Med Hyg

Specialty Tropical Medicine

Date 2009 May 30

PMID 19478259

Citations 10

Authors

Mirko Zimic

Monica Pajuelo

Daniel Rueda

Cesar Lopez

Yanina Arana

Yesenia Castillo

Maritza Calderon

Silvia Rodriguez

Patricia Sheen

Joseph M Vinetz

Armando Gonzales

Hector H Garcia

Robert H Gilman

Affiliations

Soon will be listed here.

Abstract

Neurocysticercosis, an endemic parasitic disease in most developing countries, is caused by Taenia solium and compromises the human central nervous system. Cathepsin L-like proteases are secreted by several parasites including T. solium and constitute important antigens for immunodiagnostics. A protein fraction with cathepsin L-like activity was purified from the cysticercus fluid by size exclusion and ion exchange chromatography. Cathepsin L-like activity was measured fluorometrically by detecting the hydrolysis of the fluorogenic substrate Z-Phe-Arg-AMC. The purified protein fraction included antigens of 53 and 25 kD that were tested in a Western immunoblot and in an enzyme-linked immunosorbent assay (ELISA) for detection of human cysticercosis. The sensitivity of the Western immunoblot was 96% for patients infected with multiple cysts and 78% for patients with a single cyst. Specificity was 98%. The sensitivity of the ELISA was 98% in patients with multiple cysts and 84% in patients with a single cyst. Specificity was 92.7%.

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