Crystal Structure and Association Behaviour of the GluR2 Amino-terminal Domain

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2009 May 23

PMID 19461580

Citations 98

Authors

Rongsheng Jin

Satinder K Singh

Shenyan Gu

Hiroyasu Furukawa

Alexander I Sobolevsky

Jie Zhou

Yan Jin

Eric Gouaux

Affiliations

Soon will be listed here.

Abstract

Fast excitatory neurotransmission is mediated largely by ionotropic glutamate receptors (iGluRs), tetrameric, ligand-gated ion channel proteins comprised of three subfamilies, AMPA, kainate and NMDA receptors, with each subfamily sharing a common, modular-domain architecture. For all receptor subfamilies, active channels are exclusively formed by assemblages of subunits within the same subfamily, a molecular process principally encoded by the amino-terminal domain (ATD). However, the molecular basis by which the ATD guides subfamily-specific receptor assembly is not known. Here we show that AMPA receptor GluR1- and GluR2-ATDs form tightly associated dimers and, by the analysis of crystal structures of the GluR2-ATD, propose mechanisms by which the ATD guides subfamily-specific receptor assembly.

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