Coatomer-dependent Protein Delivery to Lipid Droplets

Overview

Journal J Cell Sci

Specialty Cell Biology

Date 2009 May 23

PMID 19461073

Citations 143

Authors

Krishnakant G Soni

Gonzalo A Mardones

Rachid Sougrat

Elena Smirnova

Catherine L Jackson

Juan S Bonifacino

Affiliations

Soon will be listed here.

Abstract

Lipid droplets (LDs) are cytoplasmic organelles that store neutral lipids for use as an energy supply in times of nutrient deprivation and for membrane assembly. Misregulation of LD function leads to many human diseases, including lipodystrophy, obesity and neutral lipid storage disorders. A number of proteins have been shown to localize to the surface of lipid droplets, including lipases such as adipose triglyceride lipase (ATGL) and the PAT-domain proteins ADRP (adipophilin) and TIP47, but the mechanism by which they are targeted to LDs is not known. Here we demonstrate that ATGL and ADRP, but not TIP47, are delivered to LDs by a pathway mediated by the COPI and COPII coatomer proteins and their corresponding regulators.

Citing Articles

The evolving landscape of ER-LD contact sites.

Kumar A, Yadav S, Choudhary V Front Cell Dev Biol. 2024; 12:1483902.

PMID: 39421023 PMC: 11484260. DOI: 10.3389/fcell.2024.1483902.

Evolutionary trajectory for nuclear functions of ciliary transport complex proteins.

Ewerling A, May-Simera H Microbiol Mol Biol Rev. 2024; 88(3):e0000624.

PMID: 38995044 PMC: 11426024. DOI: 10.1128/mmbr.00006-24.

Rab1b facilitates lipid droplet growth by ER-to-lipid droplet targeting of DGAT2.

Malis Y, Armoza-Eilat S, Nevo-Yassaf I, Dukhovny A, Sklan E, Hirschberg K Sci Adv. 2024; 10(22):eade7753.

PMID: 38809969 PMC: 11135398. DOI: 10.1126/sciadv.ade7753.

Adaptations of membrane trafficking in cancer and tumorigenesis.

Evergren E, Mills I, Kennedy G J Cell Sci. 2024; 137(10).

PMID: 38770683 PMC: 11166456. DOI: 10.1242/jcs.260943.

Glucose controls lipolysis through Golgi PtdIns4P-mediated regulation of ATGL.

Ding L, Huwyler F, Long F, Yang W, Binz J, Wernle K Nat Cell Biol. 2024; 26(4):552-566.

PMID: 38561547 PMC: 11021197. DOI: 10.1038/s41556-024-01386-y.

References

Brasaemle D, Dolios G, Shapiro L, Wang R . Proteomic analysis of proteins associated with lipid droplets of basal and lipolytically stimulated 3T3-L1 adipocytes. J Biol Chem. 2004; 279(45):46835-42. DOI: 10.1074/jbc.M409340200. View

Lippincott-Schwartz J, Yuan L, Bonifacino J, Klausner R . Rapid redistribution of Golgi proteins into the ER in cells treated with brefeldin A: evidence for membrane cycling from Golgi to ER. Cell. 1989; 56(5):801-13. PMC: 7173269. DOI: 10.1016/0092-8674(89)90685-5. View

Brasaemle D . Thematic review series: adipocyte biology. The perilipin family of structural lipid droplet proteins: stabilization of lipid droplets and control of lipolysis. J Lipid Res. 2007; 48(12):2547-59. DOI: 10.1194/jlr.R700014-JLR200. View

Puertollano R, van der Wel N, Greene L, Eisenberg E, Peters P, Bonifacino J . Morphology and dynamics of clathrin/GGA1-coated carriers budding from the trans-Golgi network. Mol Biol Cell. 2003; 14(4):1545-57. PMC: 153121. DOI: 10.1091/mbc.02-07-0109. View

Nakamura N, Akashi T, Taneda T, Kogo H, Kikuchi A, Fujimoto T . ADRP is dissociated from lipid droplets by ARF1-dependent mechanism. Biochem Biophys Res Commun. 2004; 322(3):957-65. DOI: 10.1016/j.bbrc.2004.08.010. View

Arner P, Langin D . The role of neutral lipases in human adipose tissue lipolysis. Curr Opin Lipidol. 2007; 18(3):246-50. DOI: 10.1097/MOL.0b013e32811e16fb. View

Murphy D . The biogenesis and functions of lipid bodies in animals, plants and microorganisms. Prog Lipid Res. 2001; 40(5):325-438. DOI: 10.1016/s0163-7827(01)00013-3. View

Aridor M, Bannykh S, Rowe T, Balch W . Sequential coupling between COPII and COPI vesicle coats in endoplasmic reticulum to Golgi transport. J Cell Biol. 1995; 131(4):875-93. PMC: 2200014. DOI: 10.1083/jcb.131.4.875. View

Brasaemle D, Wolins N . Isolation of lipid droplets from cells by density gradient centrifugation. Curr Protoc Cell Biol. 2008; Chapter 3:Unit 3.15. DOI: 10.1002/0471143030.cb0315s29. View

10.

Magre J, Delepine M, Khallouf E, Gedde-Dahl Jr T, Van Maldergem L, Sobel E . Identification of the gene altered in Berardinelli-Seip congenital lipodystrophy on chromosome 11q13. Nat Genet. 2001; 28(4):365-70. DOI: 10.1038/ng585. View

11.

Bannykh S, Rowe T, Balch W . The organization of endoplasmic reticulum export complexes. J Cell Biol. 1996; 135(1):19-35. PMC: 2121027. DOI: 10.1083/jcb.135.1.19. View

12.

Guo Y, Walther T, Rao M, Stuurman N, Goshima G, Terayama K . Functional genomic screen reveals genes involved in lipid-droplet formation and utilization. Nature. 2008; 453(7195):657-61. PMC: 2734507. DOI: 10.1038/nature06928. View

13.

Liu P, Ying Y, Zhao Y, Mundy D, Zhu M, Anderson R . Chinese hamster ovary K2 cell lipid droplets appear to be metabolic organelles involved in membrane traffic. J Biol Chem. 2003; 279(5):3787-92. DOI: 10.1074/jbc.M311945200. View

14.

Bartz R, Zehmer J, Zhu M, Chen Y, Serrero G, Zhao Y . Dynamic activity of lipid droplets: protein phosphorylation and GTP-mediated protein translocation. J Proteome Res. 2007; 6(8):3256-65. DOI: 10.1021/pr070158j. View

15.

Bostrom P, Andersson L, Rutberg M, Perman J, Lidberg U, Johansson B . SNARE proteins mediate fusion between cytosolic lipid droplets and are implicated in insulin sensitivity. Nat Cell Biol. 2007; 9(11):1286-93. DOI: 10.1038/ncb1648. View

16.

Ward T, Polishchuk R, Caplan S, Hirschberg K, Lippincott-Schwartz J . Maintenance of Golgi structure and function depends on the integrity of ER export. J Cell Biol. 2001; 155(4):557-70. PMC: 2198855. DOI: 10.1083/jcb.200107045. View

17.

Fischer J, Lefevre C, Morava E, Mussini J, Laforet P, Negre-Salvayre A . The gene encoding adipose triglyceride lipase (PNPLA2) is mutated in neutral lipid storage disease with myopathy. Nat Genet. 2006; 39(1):28-30. DOI: 10.1038/ng1951. View

18.

Kadereit B, Kumar P, Wang W, Miranda D, Snapp E, Severina N . Evolutionarily conserved gene family important for fat storage. Proc Natl Acad Sci U S A. 2007; 105(1):94-9. PMC: 2224239. DOI: 10.1073/pnas.0708579105. View

19.

Carroll K, Hanna J, Simon I, Krise J, Barbero P, Pfeffer S . Role of Rab9 GTPase in facilitating receptor recruitment by TIP47. Science. 2001; 292(5520):1373-6. DOI: 10.1126/science.1056791. View

20.

Niu T, Pfeifer A, Lippincott-Schwartz J, Jackson C . Dynamics of GBF1, a Brefeldin A-sensitive Arf1 exchange factor at the Golgi. Mol Biol Cell. 2004; 16(3):1213-22. PMC: 551486. DOI: 10.1091/mbc.e04-07-0599. View