Collagenase Expression and Activity in the Stromal Cells from Giant Cell Tumour of Bone

Overview

Journal Bone

Specialties Endocrinology
Orthopedics

Date 2009 May 16

PMID 19442604

Citations 8

Authors

Robert W Cowan

Isabella W Y Mak

Nigel Colterjohn

Gurmit Singh

Michelle Ghert

Affiliations

Soon will be listed here.

Abstract

The characteristic bone destruction in giant cell tumour of bone (GCT) is largely attributed to the osteoclast-like giant cells. However, experimental analyses of bone resorption by cells from GCT often fail to exclude the neoplastic spindle-like stromal cells, and several studies have demonstrated that bone resorption by GCT cells is increased in the presence of stromal cells. The spindle-like stromal cells from GCT may therefore actively contribute to the bone resorption observed in the tumour. Type I collagen, a major organic constituent of bone, is effectively degraded by three matrix metalloproteinases (MMPs) known as the collagenases: MMP-1, MMP-8 and MMP-13. We established primary cell cultures from nine patients with GCT and the stromal cell populations were isolated in culture. The production of collagenases by primary cultures of GCT stromal cells was determined through real-time PCR, western blot analysis and a multiplex assay system. Results show that the cells produce MMP-1 and MMP-13 but not MMP-8. Immunohistochemistry confirmed the presence of MMP-1 and MMP-13 in paraffin-embedded GCT tissue samples. Medium conditioned by the stromal cell cultures was capable of proteolytic activity as determined by MMP-1 and MMP-13-specific standardized enzyme activity assays. The spindle-like stromal cells from GCT may therefore actively participate in the bone destruction that is characteristic of the tumour.

Citing Articles

Evaluation of the multiple tissue factors in bone of primary osteoplasty and rhinoplasty in patients affected by cleft lip palate.

Buile D, Pilmane M, Akota I Histol Histopathol. 2022; 37(7):679-690.

PMID: 35302644 DOI: 10.14670/HH-18-451.

Downregulation of ERK signaling impairs U2OS osteosarcoma cell migration in collagen matrix by suppressing MMP9 production.

Poudel B, Kim D, Ki H, Kwon Y, Lee Y, Kim D Oncol Lett. 2013; 7(1):215-218.

PMID: 24348851 PMC: 3861606. DOI: 10.3892/ol.2013.1655.

Titanium particles up-regulate the activity of matrix metalloproteinase-2 in human synovial cells.

Fu C, Xie J, Hu N, Liang X, Chen R, Wang C Int Orthop. 2013; 38(5):1091-8.

PMID: 24271334 PMC: 3997776. DOI: 10.1007/s00264-013-2190-0.

Chondroclasts are mature osteoclasts which are capable of cartilage matrix resorption.

Knowles H, Moskovsky L, Thompson M, Grunhen J, Cheng X, Kashima T Virchows Arch. 2012; 461(2):205-10.

PMID: 22782381 DOI: 10.1007/s00428-012-1274-3.

Matrix Metalloproteinase Activity in the Stromal Cell of Giant Cell Tumor of Bone.

Rabinovich A, Mak I, Cowan R, Turcotte R, Colterjohn N, Singh G Open Bone J. 2012; 1:46-52.

PMID: 22287999 PMC: 3266943. DOI: 10.2174/1876525400901010046.

References

Huang L, Teng X, Cheng Y, Lee K, Kumta S . Expression of preosteoblast markers and Cbfa-1 and Osterix gene transcripts in stromal tumour cells of giant cell tumour of bone. Bone. 2004; 34(3):393-401. DOI: 10.1016/j.bone.2003.10.013. View

Goldring S, Roelke M, Petrison K, Bhan A . Human giant cell tumors of bone identification and characterization of cell types. J Clin Invest. 1987; 79(2):483-91. PMC: 424109. DOI: 10.1172/JCI112838. View

Kanehisa J, Izumo T, Takeuchi M, Yamanaka T, Fujii T, Takeuchi H . In vitro bone resorption by isolated multinucleated giant cells from giant cell tumour of bone: light and electron microscopic study. Virchows Arch A Pathol Anat Histopathol. 1991; 419(4):327-38. DOI: 10.1007/BF01606524. View

Blair H, Teitelbaum S, Ghiselli R, Gluck S . Osteoclastic bone resorption by a polarized vacuolar proton pump. Science. 1989; 245(4920):855-7. DOI: 10.1126/science.2528207. View

Dequeker J, MERLEVEDE W . Collagen content and collagen extractability pattern of adult human trabecular bone according to age, sex and amount of bone mass. Biochim Biophys Acta. 1971; 244(2):410-20. DOI: 10.1016/0304-4165(71)90243-1. View

Lindeman J, Hanemaaijer R, Mulder A, Dijkstra P, Szuhai K, Bromme D . Cathepsin K is the principal protease in giant cell tumor of bone. Am J Pathol. 2004; 165(2):593-600. PMC: 1618565. DOI: 10.1016/S0002-9440(10)63323-8. View

Yoshida H, Akeho M, Yumoto T . Giant cell tumor bone. Enzyme histochemical, biochemical and tissue culture studies. Virchows Arch A Pathol Anat Histol. 1982; 395(3):319-30. DOI: 10.1007/BF00429357. View

Soueidan A, Gan O, Gouin F, Godard A, Heymann D, Jacques Y . Culturing of cells from giant cell tumour of bone on natural and synthetic calcified substrata: the effect of leukaemia inhibitory factor and vitamin D3 on the resorbing activity of osteoclast-like cells. Virchows Arch. 1995; 426(5):469-77. DOI: 10.1007/BF00193170. View

Benderdour M, Tardif G, Pelletier J, Di Battista J, Reboul P, Ranger P . Interleukin 17 (IL-17) induces collagenase-3 production in human osteoarthritic chondrocytes via AP-1 dependent activation: differential activation of AP-1 members by IL-17 and IL-1beta. J Rheumatol. 2002; 29(6):1262-72. View

10.

Littlewood-Evans A, Kokubo T, Ishibashi O, Inaoka T, Wlodarski B, Gallagher J . Localization of cathepsin K in human osteoclasts by in situ hybridization and immunohistochemistry. Bone. 1997; 20(2):81-6. DOI: 10.1016/s8756-3282(96)00351-1. View

11.

Fuller K, Chambers T . Localisation of mRNA for collagenase in osteocytic, bone surface and chondrocytic cells but not osteoclasts. J Cell Sci. 1995; 108 ( Pt 6):2221-30. DOI: 10.1242/jcs.108.6.2221. View

12.

Ohuchi E, Imai K, Fujii Y, Sato H, Seiki M, Okada Y . Membrane type 1 matrix metalloproteinase digests interstitial collagens and other extracellular matrix macromolecules. J Biol Chem. 1997; 272(4):2446-51. DOI: 10.1074/jbc.272.4.2446. View

13.

Oreffo R, Marshall G, Kirchen M, Garcia C, Gallwitz W, Chavez J . Characterization of a cell line derived from a human giant cell tumor that stimulates osteoclastic bone resorption. Clin Orthop Relat Res. 1993; (296):229-41. View

14.

James I, Dodds R, Eichman C, Connor J, Hart T, Maleeff B . Purification and characterization of fully functional human osteoclast precursors. J Bone Miner Res. 1996; 11(11):1608-18. DOI: 10.1002/jbmr.5650111104. View

15.

Ohsaki Y, Takahashi S, Scarcez T, Demulder A, Nishihara T, Williams R . Evidence for an autocrine/paracrine role for interleukin-6 in bone resorption by giant cells from giant cell tumors of bone. Endocrinology. 1992; 131(5):2229-34. DOI: 10.1210/endo.131.5.1425421. View

16.

Holliday L, Welgus H, Hanna J, Lee B, Lu M, Jeffrey J . Interstitial collagenase activity stimulates the formation of actin rings and ruffled membranes in mouse marrow osteoclasts. Calcif Tissue Int. 2003; 72(3):206-14. DOI: 10.1007/s00223-002-1008-7. View

17.

Nakamura H, Sato G, Hirata A, Yamamoto T . Immunolocalization of matrix metalloproteinase-13 on bone surface under osteoclasts in rat tibia. Bone. 2004; 34(1):48-56. DOI: 10.1016/j.bone.2003.09.001. View

18.

Yoon S, Park S, Yun C, Chung A . Roles of matrix metalloproteinases in tumor metastasis and angiogenesis. J Biochem Mol Biol. 2003; 36(1):128-37. DOI: 10.5483/bmbrep.2003.36.1.128. View

19.

Murata A, Fujita T, Kawahara N, Tsuchiya H, Tomita K . Osteoblast lineage properties in giant cell tumors of bone. J Orthop Sci. 2005; 10(6):581-8. DOI: 10.1007/s00776-005-0946-0. View

20.

Sasaguri Y, Komiya S, Sugama K, Suzuki K, Inoue A, Morimatsu M . Production of matrix metalloproteinases 2 and 3 (stromelysin) by stromal cells of giant cell tumor of bone. Am J Pathol. 1992; 141(3):611-21. PMC: 1886706. View