Matrix Metalloproteinase Activity in the Stromal Cell of Giant Cell Tumor of Bone

Overview

Journal Open Bone J

Publisher Bentham Science Publishers

Date 2012 Jan 31

PMID 22287999

Citations 5

Authors

Alexander Rabinovich

Isabella W Y Mak

Robert W Cowan

Robert E Turcotte

Nigel Colterjohn

Gurmit Singh

Michelle Ghert

Affiliations

Soon will be listed here.

Abstract

Giant cell tumor of bone (GCT) is a destructive and potentially metastatic bone tumour in which the characteristic giant cells have classically been considered the culprits in bone destruction. However, the neoplastic element of the tumour consists of propagative osteoblast-like stromal cells that may play a role in bone resorption. The objectives of this study were to determine the expression and activity of the gelatinases, matrix metalloproteinase (MMP)-2 and -9, in GCT stromal cells, and to determine if these cells have bone-resorbing capabilities. We performed immunohistochemistry on clinical specimens, and real-time polymerase chain reaction (PCR) and zymography on cell lysates and conditioned media from cultured clinical GCT specimens in order to evaluate the expression and activity of MMP-2 and-9 in GCT stromal cells. Our results support the fact that GCT stromal cells express MMP-2 and MMP-9 and are capable of gelatin degradation in vitro. These cells may therefore play a role in bone destruction in GCT.

Citing Articles

Denosumab in Giant Cell Tumor of Bone: Current Status and Pitfalls.

Li H, Gao J, Gao Y, Lin N, Zheng M, Ye Z Front Oncol. 2020; 10:580605.

PMID: 33123484 PMC: 7567019. DOI: 10.3389/fonc.2020.580605.

A comparative study of osteopontin and MMP-2 protein expression in peripheral and central giant cell granuloma of the jaw.

Mohtasham N, Saghravanian N, Fatemi B, Vahedi M, Afzal-Aghaee M, Kadeh H Braz J Otorhinolaryngol. 2018; 85(2):150-156.

PMID: 29339027 PMC: 9452220. DOI: 10.1016/j.bjorl.2017.11.006.

Expression of Matrix Metalloproteinase-9 and CD34 in Giant Cell Tumor of Bone.

Zhou X, Liu X, Fan G, Wu S, Zhao J, Shi X Orthop Surg. 2016; 8(2):220-5.

PMID: 27384731 PMC: 6584287. DOI: 10.1111/os.12250.

Protein Expression Profiling of Giant Cell Tumors of Bone Treated with Denosumab.

Mukaihara K, Suehara Y, Kohsaka S, Akaike K, Tanabe Y, Kubota D PLoS One. 2016; 11(2):e0148401.

PMID: 26863138 PMC: 4749282. DOI: 10.1371/journal.pone.0148401.

Investigation of FGFR2-IIIC signaling via FGF-2 ligand for advancing GCT stromal cell differentiation.

Singh S, Singh M, Mak I, Turcotte R, Ghert M PLoS One. 2012; 7(10):e46769.

PMID: 23071632 PMC: 3469652. DOI: 10.1371/journal.pone.0046769.

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