Antioxidative Activity of Polysaccharide Fractions Isolated from Lycium Barbarum Linnaeus

Overview

Journal Int J Biol Macromol

Publisher Elsevier

Date 2009 May 5

PMID 19409411

Citations 42

Authors

C L Lin

C C Wang

S C Chang

B Stephen Inbaraj

B H Chen

Affiliations

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Abstract

Antioxidant activity of polysaccharide fractions isolated from Lycium barbarum Linnaeus was evaluated. Polysaccharides were extracted with boiling water, followed by precipitating with ethanol, protein hydrolysis, dialysis, and fractionation with a DEAE-Sepharose CL-6B column. A total of 4 fractions, including 1 neutral polysaccharide (LBPN) and 3 acidic polysaccharides were obtained, and compared with crude polysaccharide (CP), crude extract of polysaccharide (CE), deproteinated polysaccharide (DP), and deproteinated and dialyzed polysaccharide (DDP) for antioxidative activity. With the exception of CE and DDP, most polysaccharides were effective in scavenging DPPH and ABTS(.)+ free radicals, superoxide anion and hydroxyl radical at 1000 microg/mL.

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