High-throughput Generation of Tagged Stable Cell Lines for Proteomic Analysis

Overview

Journal Proteomics

Date 2009 May 1

PMID 19405035

Citations 74

Authors

Jorge Z Torres

Julie J Miller

Peter K Jackson

Affiliations

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Abstract

We present an optimized system for rapid generation of localization and affinity purification-tagged mammalian stable cell lines that facilitates complex purification and interacting protein identification. The improved components of this method, including the flexibility of inducible expression, circumvent issues associated with toxicity, clonal selection, sample yields and time to data acquisition. We have applied this method to the study of cell-cycle regulators and novel microtubule-associated proteins.

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