HORFeome V3.1: a Resource of Human Open Reading Frames Representing over 10,000 Human Genes

Overview

Journal Genomics

Specialty Genetics

Date 2007 Jan 9

PMID 17207965

Citations 155

Authors

Philippe Lamesch

Ning Li

Stuart Milstein

Changyu Fan

Tong Hao

Gabor Szabo

Zhenjun Hu

Kavitha Venkatesan

Graeme Bethel

Paul Martin

Jane Rogers

Stephanie Lawlor

Stuart McLaren

Amelie Dricot

Heather Borick

Michael E Cusick

Jean Vandenhaute

Ian Dunham

David E Hill

Marc Vidal

Affiliations

Soon will be listed here.

Abstract

Complete sets of cloned protein-encoding open reading frames (ORFs), or ORFeomes, are essential tools for large-scale proteomics and systems biology studies. Here we describe human ORFeome version 3.1 (hORFeome v3.1), currently the largest publicly available resource of full-length human ORFs (available at ). Generated by Gateway recombinational cloning, this collection contains 12,212 ORFs, representing 10,214 human genes, and corresponds to a 51% expansion of the original hORFeome v1.1. An online human ORFeome database, hORFDB, was built and serves as the central repository for all cloned human ORFs (http://horfdb.dfci.harvard.edu). This expansion of the original ORFeome resource greatly increases the potential experimental search space for large-scale proteomics studies, which will lead to the generation of more comprehensive datasets.

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