Evidence for Persistent Hepatitis C Virus (HCV) Infection in Hemophiliacs

Overview

Journal J Clin Invest

Specialty General Medicine

Date 1991 Nov 1

PMID 1939652

Citations 5

Authors

J P Allain

S H Dailey

Y Laurian

D S Vallari

A Rafowicz

S M Desai

S G Devare

Affiliations

Soon will be listed here.

Abstract

Hepatitis C virus (HCV) is the major etiologic agent associated with non-A, non-B hepatitis. This study was designed to assess virologic and serologic markers in hemophiliacs exposed to non-heat-treated and/or virus-inactivated plasma derivatives. Serial bleeds from 48 hemophilic patients were analyzed for the presence of HCV viral RNA sequences as detected by polymerase chain reaction (PCR) and antibodies to structural (core) and nonstructural (C-100 and 33C) proteins by specific dot immunoblot assay. All patients exposed to non-heat-treated products, and four of six patients exposed only to virus inactivated products, had evidence of HCV infection. However, over the 5-yr study period, six exposed patients (13%) consistently lacked detectable anti-C-100 and seven (15%) lost this antibody. HCV viremia (PCR positive) was found in 91% of exposed patients, and was significantly more frequent in HIV seropositive hemophiliacs (P less than 0.05). Six patients had high antibody level to HCV and elevated ALT, but appeared to clear viremia. Four hemophiliacs were HCV seropositive but lacked detectable viremia. These data indicate that hemophiliacs remain persistently infected by HCV and that antibody to the core antigen of HCV is a reliable marker of this transfusion transmissible agent.

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References

Preston F, Hay C, Dewar M, Greaves M, Triger D . Non-A, non-B hepatitis and heat-treated factor VIII concentrates. Lancet. 1985; 2(8448):213. DOI: 10.1016/s0140-6736(85)91525-9. View

Wang J, Wang T, Lin J, Sheu J, Lin S, Chen D . Hepatitis C virus RNA in saliva of patients with post-transfusion hepatitis C infection. Lancet. 1991; 337(8732):48. DOI: 10.1016/0140-6736(91)93365-g. View

Fine J, Allain J, Rochu D, Marneux M . Occurrence of normal circulating proalbumin in a hemophilic A patient after acute hepatitis related to the delta virus. Clin Chem. 1986; 32(10):1991-3. View

Schimpf K, Mannucci P, Kreutz W, Brackmann H, Auerswald G, Ciavarella N . Absence of hepatitis after treatment with a pasteurized factor VIII concentrate in patients with hemophilia and no previous transfusions. N Engl J Med. 1987; 316(15):918-22. DOI: 10.1056/NEJM198704093161505. View

HOROWITZ M, Rooks C, Horowitz B, Hilgartner M . Virus safety of solvent/detergent-treated antihaemophilic factor concentrate. Lancet. 1988; 2(8604):186-9. DOI: 10.1016/s0140-6736(88)92288-x. View

Lai M, Farci P, Figus A, Balestrieri A, Arnone M, VYAS G . Hepatitis B virus DNA in the serum of Sardinian blood donors negative for the hepatitis B surface antigen. Blood. 1989; 73(1):17-9. View

Esteban J, Esteban R, Viladomiu L, Lopez-Talavera J, Gonzalez A, Hernandez J . Hepatitis C virus antibodies among risk groups in Spain. Lancet. 1989; 2(8658):294-7. DOI: 10.1016/s0140-6736(89)90485-6. View

. Antibodies to hepatitis C virus in haemophilia. Lancet. 1989; 2(8662):560-1. View

Donohue J, Bailey M, Gray R, Holen J, Huang T, Keevan J . Enzyme immunoassay system for panel testing. Clin Chem. 1989; 35(9):1874-7. View

10.

Colombo M, Mannucci P, Carnelli V, Savidge G, Gazengel C, Schimpf K . Transmission of non-A, non-B hepatitis by heat-treated factor VIII concentrate. Lancet. 1985; 2(8445):1-4. DOI: 10.1016/s0140-6736(85)90055-8. View

11.

Coombs R, Collier A, Allain J, Nikora B, Leuther M, Gjerset G . Plasma viremia in human immunodeficiency virus infection. N Engl J Med. 1989; 321(24):1626-31. DOI: 10.1056/NEJM198912143212402. View

12.

Kubo Y, Takeuchi K, Boonmar S, Katayama T, Choo Q, Kuo G . A cDNA fragment of hepatitis C virus isolated from an implicated donor of post-transfusion non-A, non-B hepatitis in Japan. Nucleic Acids Res. 1989; 17(24):10367-72. PMC: 335306. DOI: 10.1093/nar/17.24.10367. View

13.

Weiner A, Kuo G, Bradley D, Bonino F, Saracco G, Lee C . Detection of hepatitis C viral sequences in non-A, non-B hepatitis. Lancet. 1990; 335(8680):1-3. DOI: 10.1016/0140-6736(90)90134-q. View

14.

Rumi M, Colombo M, Gringeri A, Mannucci P . High prevalence of antibody to hepatitis C virus in multitransfused hemophiliacs with normal transaminase levels. Ann Intern Med. 1990; 112(5):379-80. DOI: 10.7326/0003-4819-112-5-379. View

15.

Makris M, Preston F, Triger D, Underwood J, Choo Q, Kuo G . Hepatitis C antibody and chronic liver disease in haemophilia. Lancet. 1990; 335(8698):1117-9. DOI: 10.1016/0140-6736(90)91124-s. View

16.

Garson J, Preston F, Makris M, Tuke P, Ring C, Machin S . Detection by PCR of hepatitis C virus in factor VIII concentrates. Lancet. 1990; 335(8703):1473. DOI: 10.1016/0140-6736(90)91510-h. View

17.

Bolling T, Mandecki W . An Escherichia coli expression vector for high-level production of heterologous proteins in fusion with CMP-KDO synthetase. Biotechniques. 1990; 8(5):488-92. View

18.

Brettler D, Alter H, Dienstag J, Forsberg A, Levine P . Prevalence of hepatitis C virus antibody in a cohort of hemophilia patients. Blood. 1990; 76(1):254-6. View

19.

Boudart D, Lucas J, Muller J, Le Carrer D, Planchon B, Harousseau J . False-positive hepatitis C virus antibody tests in paraproteinaemia. Lancet. 1990; 336(8706):63. DOI: 10.1016/0140-6736(90)91585-x. View

20.

Laurence J . Molecular interactions among herpesviruses and human immunodeficiency viruses. J Infect Dis. 1990; 162(2):338-46. DOI: 10.1093/infdis/162.2.338. View