Serology and Leprosy: Immunoassays Comparing Immunoglobulin G Antibody Responses to 28- and 30-kilodalton Proteins Purified from Mycobacterium Bovis BCG

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1991 Oct 1

PMID 1939585

Citations 4

Authors

M C Pessolani

J M Peralta

F D Rumjanek

H M Gomes

M A Marques

E C Almeida

M H Saad

E N Sarno

Affiliations

Soon will be listed here.

Abstract

Two major proteins from Mycobacterium bovis BCG culture filtrates with molecular masses of 28 kDa (P28) and 30 kDa (P30), identified as components of the BCG 85 complex, were purified and used in enzyme-linked immunosorbent assays (ELISAs) for the determination of specific immunoglobulin G (IgG) levels in patients with leprosy or tuberculosis or with exposure to these diseases. High reactivity to both antigens was observed with sera from lepromatous leprosy patients, whereas antibody levels in sera from paucibacillary leprosy patients were not significantly different from those in sera from healthy individuals from an area in which leprosy is endemic. High IgG responses were also found in some contacts of lepromatous leprosy patients. A comparison of the levels of anti-P28 and anti-P30 within the multibacillary leprosy patient group showed much higher IgG reactivity to P28 than to P30, suggesting that the antibody response of lepromatous patients is directed predominantly against the 28-kDa protein. A high degree of correlation in values of ELISAs based on P28 and on the phenolic glycolipid of Mycobacterium leprae was observed in all groups analyzed. The potential use of an assay based on the 28-kDa protein to selectively distinguish individuals destined to develop multibacillary leprosy is discussed, as also is the likelihood that the 28-kDa-30-kDa complex, part of the fibronectin-binding family, is an important component of M. leprae.

Citing Articles

Modulation of humoral immune response to oral BCG vaccination by Mycobacterium bovis BCG Moreau Rio de Janeiro (RDJ) in healthy adults.

Monteiro-Maia R, Ortigao-de-Sampaio M, Pinho R, Castello-Branco L J Immune Based Ther Vaccines. 2006; 4:4.

PMID: 16956404 PMC: 1569376. DOI: 10.1186/1476-8518-4-4.

Purification and immunoreactivity of three components from the 30/32-kilodalton antigen 85 complex in Mycobacterium tuberculosis.

Lim J, Park J, Jo E, Song C, Min D, Song Y Infect Immun. 1999; 67(11):6187-90.

PMID: 10531287 PMC: 97013. DOI: 10.1128/IAI.67.11.6187-6190.1999.

Humoral responses against the 85A and 85B antigens of Mycobacterium bovis BCG in patients with leprosy and tuberculosis.

van Vooren J, Drowart A, de Bruyn J, Launois P, Millan J, Delaporte E J Clin Microbiol. 1992; 30(6):1608-10.

PMID: 1624586 PMC: 265344. DOI: 10.1128/jcm.30.6.1608-1610.1992.

Mycobacterium leprae produces extracellular homologs of the antigen 85 complex.

Pessolani M, Brennan P Infect Immun. 1992; 60(11):4452-9.

PMID: 1398959 PMC: 258188. DOI: 10.1128/iai.60.11.4452-4459.1992.

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