Purification and Immunoreactivity of Three Components from the 30/32-kilodalton Antigen 85 Complex in Mycobacterium Tuberculosis

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1999 Oct 26

PMID 10531287

Citations 18

Authors

J H Lim

J K Park

E K Jo

C H Song

D Min

Y J Song

H J Kim

Affiliations

Soon will be listed here.

Abstract

The three proteins of the antigen 85 complex (85A, 85B, and 85C), which are major secretory products of Mycobacterium tuberculosis, were purified to homogeneity in large amounts by a combination of chromatography on hydroxylapatite, DEAE-Sepharose, and DEAE-Sephacel and gel filtration from M. tuberculosis culture filtrate. Then we examined the immunological reactivity of the three proteins in tuberculosis patients and healthy controls. Antibody responses to the 85B and 85A proteins in patients were significantly greater than responses to the 85C protein. In contrast, all three antigens induced significant lymphoproliferation and gamma interferon production in peripheral blood mononuclear cells from healthy tuberculin reactors.

Citing Articles

Antibody Fc Glycosylation Discriminates Between Latent and Active Tuberculosis.

Lu L, Das J, Grace P, Fortune S, Restrepo B, Alter G J Infect Dis. 2020; 222(12):2093-2102.

PMID: 32060529 PMC: 7661770. DOI: 10.1093/infdis/jiz643.

Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications.

Russell T, Green L, Rice T, Kruh-Garcia N, Dobos K, De Groote M J Clin Microbiol. 2017; 55(10):3072-3088.

PMID: 28794178 PMC: 5625393. DOI: 10.1128/JCM.00469-17.

Mycobacterium avium MAV2054 protein induces macrophage apoptosis by targeting mitochondria and reduces intracellular bacterial growth.

Lee K, Whang J, Choi H, Son Y, Jeon H, Back Y Sci Rep. 2016; 6:37804.

PMID: 27901051 PMC: 5129020. DOI: 10.1038/srep37804.

Microchip-based ultrafast serodiagnostic assay for tuberculosis.

Mani V, Paleja B, Larbi K, Kumar P, Tay J, Siew J Sci Rep. 2016; 6:35845.

PMID: 27775039 PMC: 5075771. DOI: 10.1038/srep35845.

The viability and protein expression of Beijing/W lineage Mycobacterium tuberculosis circulating in Xinjiang, China.

Li F, Li H, Zuo W, Mi L, Wang X, Wang Y Curr Microbiol. 2015; 70(5):735-44.

PMID: 25656263 PMC: 4377140. DOI: 10.1007/s00284-015-0776-z.

References

Wiker H, HARBOE M, NAGAI S, Bennedsen J . Quantitative and qualitative studies on the major extracellular antigen of Mycobacterium tuberculosis H37Rv and Mycobacterium bovis BCG. Am Rev Respir Dis. 1990; 141(4 Pt 1):830-8. DOI: 10.1164/ajrccm/141.4_Pt_1.830. View

de Bruyn J, Huygen K, Bosmans R, Fauville M, Lippens R, van Vooren J . Purification, characterization and identification of a 32 kDa protein antigen of Mycobacterium bovis BCG. Microb Pathog. 1987; 2(5):351-66. DOI: 10.1016/0882-4010(87)90077-5. View

Havlir D, Wallis R, Boom W, Daniel T, Chervenak K, Ellner J . Human immune response to Mycobacterium tuberculosis antigens. Infect Immun. 1991; 59(2):665-70. PMC: 257808. DOI: 10.1128/iai.59.2.665-670.1991. View

Content J, de la Cuvellerie A, De Wit L, Ooms J, de Bruyn J . The genes coding for the antigen 85 complexes of Mycobacterium tuberculosis and Mycobacterium bovis BCG are members of a gene family: cloning, sequence determination, and genomic organization of the gene coding for antigen 85-C of M. tuberculosis. Infect Immun. 1991; 59(9):3205-12. PMC: 258154. DOI: 10.1128/iai.59.9.3205-3212.1991. View

Pessolani M, Peralta J, Rumjanek F, Gomes H, Marques M, Almeida E . Serology and leprosy: immunoassays comparing immunoglobulin G antibody responses to 28- and 30-kilodalton proteins purified from Mycobacterium bovis BCG. J Clin Microbiol. 1991; 29(10):2285-90. PMC: 270314. DOI: 10.1128/jcm.29.10.2285-2290.1991. View

van Vooren J, Drowart A, De Cock M, Van Onckelen A, DHoop M, Yernault J . Humoral immune response of tuberculous patients against the three components of the Mycobacterium bovis BCG 85 complex separated by isoelectric focusing. J Clin Microbiol. 1991; 29(10):2348-50. PMC: 270329. DOI: 10.1128/jcm.29.10.2348-2350.1991. View

Schoel B, Gulle H, Kaufmann S . Heterogeneity of the repertoire of T cells of tuberculosis patients and healthy contacts to Mycobacterium tuberculosis antigens separated by high-resolution techniques. Infect Immun. 1992; 60(4):1717-20. PMC: 257056. DOI: 10.1128/iai.60.4.1717-1720.1992. View

van Vooren J, Drowart A, de Bruyn J, Launois P, Millan J, Delaporte E . Humoral responses against the 85A and 85B antigens of Mycobacterium bovis BCG in patients with leprosy and tuberculosis. J Clin Microbiol. 1992; 30(6):1608-10. PMC: 265344. DOI: 10.1128/jcm.30.6.1608-1610.1992. View

Wiker H, HARBOE M . The antigen 85 complex: a major secretion product of Mycobacterium tuberculosis. Microbiol Rev. 1992; 56(4):648-61. PMC: 372892. DOI: 10.1128/mr.56.4.648-661.1992. View

10.

Launois P, DeLeys R, Niang M, Drowart A, Andrien M, Dierckx P . T-cell-epitope mapping of the major secreted mycobacterial antigen Ag85A in tuberculosis and leprosy. Infect Immun. 1994; 62(9):3679-87. PMC: 303018. DOI: 10.1128/iai.62.9.3679-3687.1994. View

11.

Horwitz M, Lee B, DILLON B, Harth G . Protective immunity against tuberculosis induced by vaccination with major extracellular proteins of Mycobacterium tuberculosis. Proc Natl Acad Sci U S A. 1995; 92(5):1530-4. PMC: 42553. DOI: 10.1073/pnas.92.5.1530. View

12.

Silver R, Wallis R, Ellner J . Mapping of T cell epitopes of the 30-kDa alpha antigen of Mycobacterium bovis strain bacillus Calmette-Guérin in purified protein derivative (PPD)-positive individuals. J Immunol. 1995; 154(9):4665-74. View

13.

Harth G, Lee B, Wang J, Clemens D, Horwitz M . Novel insights into the genetics, biochemistry, and immunocytochemistry of the 30-kilodalton major extracellular protein of Mycobacterium tuberculosis. Infect Immun. 1996; 64(8):3038-47. PMC: 174185. DOI: 10.1128/iai.64.8.3038-3047.1996. View

14.

Garbe T, Hibler N, Deretic V . Isoniazid induces expression of the antigen 85 complex in Mycobacterium tuberculosis. Antimicrob Agents Chemother. 1996; 40(7):1754-6. PMC: 163413. DOI: 10.1128/AAC.40.7.1754. View

15.

Lozes E, Huygen K, Content J, Denis O, Montgomery D, Yawman A . Immunogenicity and efficacy of a tuberculosis DNA vaccine encoding the components of the secreted antigen 85 complex. Vaccine. 1997; 15(8):830-3. DOI: 10.1016/s0264-410x(96)00274-5. View

16.

Torres M, Herrera T, VILLAREAL H, Rich E, Sada E . Cytokine profiles for peripheral blood lymphocytes from patients with active pulmonary tuberculosis and healthy household contacts in response to the 30-kilodalton antigen of Mycobacterium tuberculosis. Infect Immun. 1998; 66(1):176-80. PMC: 107874. DOI: 10.1128/IAI.66.1.176-180.1998. View

17.

Lyashchenko K, Colangeli R, Houde M, Al Jahdali H, Menzies D, Gennaro M . Heterogeneous antibody responses in tuberculosis. Infect Immun. 1998; 66(8):3936-40. PMC: 108457. DOI: 10.1128/IAI.66.8.3936-3940.1998. View

18.

Quan X, Newman T, Huygen K, Godfrey H . Pathophysiology of antigen 85 in patients with active tuberculosis: antigen 85 circulates as complexes with fibronectin and immunoglobulin G. Infect Immun. 1999; 67(2):581-8. PMC: 96358. DOI: 10.1128/IAI.67.2.581-588.1999. View

19.

Lee B, Horwitz M . T-cell epitope mapping of the three most abundant extracellular proteins of Mycobacterium tuberculosis in outbred guinea pigs. Infect Immun. 1999; 67(5):2665-70. PMC: 116023. DOI: 10.1128/IAI.67.5.2665-2670.1999. View

20.

Kim H, Jo E, Park J, Lim J, Min D, Paik T . Isolation and partial characterisation of the Triton X-100 solubilised protein antigen from Mycobacterium tuberculosis. J Med Microbiol. 1999; 48(6):585-591. DOI: 10.1099/00222615-48-6-585. View