Genetic Polymorphisms of FSHR, CYP17, CYP1A1, CAPN10, INSR, SERPINE1 Genes in Adolescent Girls with Polycystic Ovary Syndrome

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2009 Apr 24

PMID 19387820

Citations 48

Authors

Tugba Unsal

Ece Konac

Ediz Yesilkaya

Akin Yilmaz

Aysun Bideci

Hacer Ilke Onen

Peyami Cinaz

Adnan Menevse

Affiliations

Soon will be listed here.

Abstract

Background: Polycystic ovary syndrome (PCOS), whose genetic basis is not completely well understood, is the most common endocrine disorder in women and it typically develops during adolescence. The aim of this study is to investigate the possible association between single nucleotide polymorphisms (SNPs) of FSHR, CYP17, CYP1A1, CAPN10, INSR, SERPINE1 genes and PCOS in adolescent girls.

Methods: DNA samples from forty-four adolescent girls with PCOS and 50 healthy controls were analyzed by PCR-RFLP and direct DNA sequencing to determine the genotypic frequency of 17 different polymorphic loci on the FSHR (A307T, N680S), CYP17 (-34 T/C), CYP1A1 (T6235C), CAPN10 (44, 43, 19, 63), INSR (exon 17 C/T), SERPINE1 (4G/5G) genes. Genotyping of exon 12 (six polymorphisms) and intron 12 (one polymorphism) of INSR gene by direct DNA sequencing was performed for the first time in this study.

Results: No significant differences were observed in the genotype and allele distributions of above mentioned polymorphisms between cases and control groups.

Conclusion: Our data does not support an association between SNPs of FSHR, CYP17, CYP1A1, CAPN10, INSR, SERPINE1 genes and susceptibility to PCOS or related traits in Turkish adolescent girls.

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